The effect of ATM kinase inhibition on the initial response of human dental pulp and periodontal ligament mesenchymal stem cells to ionizing radiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896447" target="_blank" >RIV/00216275:25310/13:39896447 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/13:10174041 RIV/60162694:G44__/13:43874919

Výsledek na webu

<a href="http://informahealthcare.com/doi/abs/10.3109/09553002.2013.775530" target="_blank" >http://informahealthcare.com/doi/abs/10.3109/09553002.2013.775530</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3109/09553002.2013.775530" target="_blank" >10.3109/09553002.2013.775530</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of ATM kinase inhibition on the initial response of human dental pulp and periodontal ligament mesenchymal stem cells to ionizing radiation

Popis výsledku v původním jazyce

Purpose : This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after gamma-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. Methods : MSC were irradiatedwith 2 and 20 Gy by Co-60. For ATM inhibition, specific inhibitor KU55933 was used. DNA damage was measured by Comet assay and gamma H2AX detection. Cell cycle distribution and proteins responding to DNA damage were analyzed 2-72 h after the irradiation.Results : The irradiation of MSC causes an increase in gamma H2AX; the phosphorylation was ATM-dependent. Irradiation activates ATM kinase, and the level of p53 protein is increased due to its phosphorylation on serine15. While this phosphorylation of p53 is ATM-dependent in MSC, the increase in p53 was not prevented by ATM inhibition. A similar trend was observed for Chk1 and Chk2. The increase in p21 is greater without ATM inhibition. ATM inhibition also does not fully abrogate the ac

Název v anglickém jazyce

The effect of ATM kinase inhibition on the initial response of human dental pulp and periodontal ligament mesenchymal stem cells to ionizing radiation

Popis výsledku anglicky

Purpose : This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after gamma-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. Methods : MSC were irradiatedwith 2 and 20 Gy by Co-60. For ATM inhibition, specific inhibitor KU55933 was used. DNA damage was measured by Comet assay and gamma H2AX detection. Cell cycle distribution and proteins responding to DNA damage were analyzed 2-72 h after the irradiation.Results : The irradiation of MSC causes an increase in gamma H2AX; the phosphorylation was ATM-dependent. Irradiation activates ATM kinase, and the level of p53 protein is increased due to its phosphorylation on serine15. While this phosphorylation of p53 is ATM-dependent in MSC, the increase in p53 was not prevented by ATM inhibition. A similar trend was observed for Chk1 and Chk2. The increase in p21 is greater without ATM inhibition. ATM inhibition also does not fully abrogate the ac

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA304%2F09%2F1568" target="_blank" >GA304/09/1568: Charakteristika odolnosti dospělých kmenových buněk vůči genotoxickému stresu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Radiation Biology

ISSN

0955-3002

e-ISSN

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Svazek periodika

89

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

501-511

Kód UT WoS článku

000321146200003

EID výsledku v databázi Scopus

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