HPLC determination of arginases inhibitor N-(omega)-hydroxy-nor-L-arginine using core-shell particle column and LC-MS/MS identification of principal metabolite in rat plasma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10105402" target="_blank" >RIV/00216208:11150/12:10105402 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/12:10105402

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S1570023211007537" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1570023211007537</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2011.11.022" target="_blank" >10.1016/j.jchromb.2011.11.022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HPLC determination of arginases inhibitor N-(omega)-hydroxy-nor-L-arginine using core-shell particle column and LC-MS/MS identification of principal metabolite in rat plasma

Popis výsledku v původním jazyce

For the purpose of in vivo pharmacokinetic studies, an HPLC method was developed and validated for the quantification of N-(omega)-hydroxy-nor-L-arginine, L-arginine and N-(omega)-ethyl-L-arginine (internal standard) in rat plasma. Sample processing involved a solid-phase extraction on the Waters MCX cartridges and on-line pre-column derivatization of the analytes with o-phthaldialdehyde and 3-mercaptopropionic acid. Separation of the derivatives was carried out on a core-shell Kinetex C18 column in a gradient elution mode with a mobile phase consisting of methanol and water (pH = 3.00 adjusted with formic acid). Fluorimetric detection with the excitation/emission wavelengths of 235/450 nm was used. The method was validated according to the FDA guidelines and applied to pilot pharmacokinetic experiments. An unknown metabolite was extracted from the plasma of Wistar rats after a single bolus of N-(omega)-hydroxy-nor-L-arginine (iv. 10 mg kg(-1)). The metabolite was identified as nor-L-a

Název v anglickém jazyce

HPLC determination of arginases inhibitor N-(omega)-hydroxy-nor-L-arginine using core-shell particle column and LC-MS/MS identification of principal metabolite in rat plasma

Popis výsledku anglicky

For the purpose of in vivo pharmacokinetic studies, an HPLC method was developed and validated for the quantification of N-(omega)-hydroxy-nor-L-arginine, L-arginine and N-(omega)-ethyl-L-arginine (internal standard) in rat plasma. Sample processing involved a solid-phase extraction on the Waters MCX cartridges and on-line pre-column derivatization of the analytes with o-phthaldialdehyde and 3-mercaptopropionic acid. Separation of the derivatives was carried out on a core-shell Kinetex C18 column in a gradient elution mode with a mobile phase consisting of methanol and water (pH = 3.00 adjusted with formic acid). Fluorimetric detection with the excitation/emission wavelengths of 235/450 nm was used. The method was validated according to the FDA guidelines and applied to pilot pharmacokinetic experiments. An unknown metabolite was extracted from the plasma of Wistar rats after a single bolus of N-(omega)-hydroxy-nor-L-arginine (iv. 10 mg kg(-1)). The metabolite was identified as nor-L-a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

—

Svazek periodika

880

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

90-99

Kód UT WoS článku

000299197600013

EID výsledku v databázi Scopus

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