Parallel treatment with valproic acid and all-trans retinoic acid does not potentiate P21 expression in U-937 PML/RAR cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10133717" target="_blank" >RIV/00216208:11150/12:10133717 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/12:43874776

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Parallel treatment with valproic acid and all-trans retinoic acid does not potentiate P21 expression in U-937 PML/RAR cells

Popis výsledku v původním jazyce

We studied effect of valproic acid, a representative of histondeacetylases inhibitors, on cell cycle and protein expression in U-937 PML/RAR cells of human histiocytic lymphoma. Moreover, we tried to potentiate its effect by concomitant treatment with the differentiation agent all-trans retinoic acid. We proved acetylation of nuclear histones H3 and H4 after VA treatment. Activation of non-histone proteins (as p21) preceded this acetylation. Protein p21 as an inhibitor of cyclin dependent kinases causescell cycle arrest in G1, resulting in a decrease of the percentage of cells in the S phase, as well as in the inhibition of the proliferation, which we demonstrated after VA treatment. Using concomitant treatment with VA and ATRA failed to yield a synergistic or additive effect on p21 expression in our experiments. Thus different treatment schedules should be the aim of further studies.

Název v anglickém jazyce

Parallel treatment with valproic acid and all-trans retinoic acid does not potentiate P21 expression in U-937 PML/RAR cells

Popis výsledku anglicky

We studied effect of valproic acid, a representative of histondeacetylases inhibitors, on cell cycle and protein expression in U-937 PML/RAR cells of human histiocytic lymphoma. Moreover, we tried to potentiate its effect by concomitant treatment with the differentiation agent all-trans retinoic acid. We proved acetylation of nuclear histones H3 and H4 after VA treatment. Activation of non-histone proteins (as p21) preceded this acetylation. Protein p21 as an inhibitor of cyclin dependent kinases causescell cycle arrest in G1, resulting in a decrease of the percentage of cells in the S phase, as well as in the inhibition of the proliferation, which we demonstrated after VA treatment. Using concomitant treatment with VA and ATRA failed to yield a synergistic or additive effect on p21 expression in our experiments. Thus different treatment schedules should be the aim of further studies.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plzeňský lékařský sborník

ISSN

0551-1038

e-ISSN

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Svazek periodika

78

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

9-14

Kód UT WoS článku

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EID výsledku v databázi Scopus

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