Development of an HPLC fluorescence method for determination of boldine in plasma, bile and urine of rats and identification of its major metabolites by LC-MS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F13%3A10140008" target="_blank" >RIV/00216208:11150/13:10140008 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jchromb.2013.07.009" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2013.07.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2013.07.009" target="_blank" >10.1016/j.jchromb.2013.07.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of an HPLC fluorescence method for determination of boldine in plasma, bile and urine of rats and identification of its major metabolites by LC-MS/MS

Popis výsledku v původním jazyce

Boldine belongs to the group of aporphine alkaloids isolated from Boldo tree. In contrast with numerous reports on the pharmacological effects of boldine, the data about its pharmacokinetics and biotransformation are scarce. No validated bioanalytical method of sufficient sensitivity has so far been described in the literature which could be used for quantification of boldine in various body fluids collected in pharmacokinetic studies. This work presents, for the first time, the assay for boldine in theplasma, bile and urine of rats. It includes liquid-liquid extraction/back-extraction of boldine, its chromatographic separation and sensitive fluorescence detection. Separation was carried out on a pentafluorophenyl core-shell column (Kinetex PFP, 150 x3 mm, 2.6 ?m) in gradient elution mode with solvent system consisting of an acetonitrile-ammonium formate buffer (5 mM, pH = 3.8). Fluorimetric detection (?EX = 320 nm, ?EM = 370 nm) was used for quantitative work. Validation according t

Název v anglickém jazyce

Development of an HPLC fluorescence method for determination of boldine in plasma, bile and urine of rats and identification of its major metabolites by LC-MS/MS

Popis výsledku anglicky

Boldine belongs to the group of aporphine alkaloids isolated from Boldo tree. In contrast with numerous reports on the pharmacological effects of boldine, the data about its pharmacokinetics and biotransformation are scarce. No validated bioanalytical method of sufficient sensitivity has so far been described in the literature which could be used for quantification of boldine in various body fluids collected in pharmacokinetic studies. This work presents, for the first time, the assay for boldine in theplasma, bile and urine of rats. It includes liquid-liquid extraction/back-extraction of boldine, its chromatographic separation and sensitive fluorescence detection. Separation was carried out on a pentafluorophenyl core-shell column (Kinetex PFP, 150 x3 mm, 2.6 ?m) in gradient elution mode with solvent system consisting of an acetonitrile-ammonium formate buffer (5 mM, pH = 3.8). Fluorimetric detection (?EX = 320 nm, ?EM = 370 nm) was used for quantitative work. Validation according t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

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Svazek periodika

936

Číslo periodika v rámci svazku

1 October

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

48-56

Kód UT WoS článku

000324564400007

EID výsledku v databázi Scopus

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