Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F14%3A10210604" target="_blank" >RIV/00216208:11150/14:10210604 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs10620-013-2966-5" target="_blank" >http://link.springer.com/article/10.1007%2Fs10620-013-2966-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10620-013-2966-5" target="_blank" >10.1007/s10620-013-2966-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats

Popis výsledku v původním jazyce

Background: Two-thirds partial hepatectomy (PHx) is an established model for the study of liver regeneration after resection. This process is accompanied by oxidative stress. Aims: In our study, we tested the effect of epigallocatechin gallate (EGCG), agreen tea antioxidant, on the early phase of liver regeneration after PHx. Methods: Male Wistar rats were divided into 5 groups: I) laparotomy + water for injections intraperitoneally, II) laparotomy + EGCG 50 mg/kg body weight, III) PHx + water for injections, IV) PHx + EGCG 20 mg/kg, V) PHx + EGCG 50 mg/kg, during 3 consecutive days. The rats were sacrificed 24 hours after surgery. Biochemical analysis of rat sera was performed. Histological samples were stained with hematoxyline-eosine and bromodeoxyuridine (BrdU). In hepatectomized rats, we also measured plasma malondialdehyde, tissue malondialdehyde, glutathione and cytokines levels, the activity of caspases 3/7, expression of genes Nqo-1 and HO-1 at mRNA level, expression of genes

Název v anglickém jazyce

Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats

Popis výsledku anglicky

Background: Two-thirds partial hepatectomy (PHx) is an established model for the study of liver regeneration after resection. This process is accompanied by oxidative stress. Aims: In our study, we tested the effect of epigallocatechin gallate (EGCG), agreen tea antioxidant, on the early phase of liver regeneration after PHx. Methods: Male Wistar rats were divided into 5 groups: I) laparotomy + water for injections intraperitoneally, II) laparotomy + EGCG 50 mg/kg body weight, III) PHx + water for injections, IV) PHx + EGCG 20 mg/kg, V) PHx + EGCG 50 mg/kg, during 3 consecutive days. The rats were sacrificed 24 hours after surgery. Biochemical analysis of rat sera was performed. Histological samples were stained with hematoxyline-eosine and bromodeoxyuridine (BrdU). In hepatectomized rats, we also measured plasma malondialdehyde, tissue malondialdehyde, glutathione and cytokines levels, the activity of caspases 3/7, expression of genes Nqo-1 and HO-1 at mRNA level, expression of genes

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Digestive Diseases and Sciences

ISSN

0163-2116

e-ISSN

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Svazek periodika

59

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

976-985

Kód UT WoS článku

000334748000015

EID výsledku v databázi Scopus

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