The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F14%3A10218611" target="_blank" >RIV/00216208:11150/14:10218611 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.nrcresearchpress.com/doi/abs/10.1139/cjpp-2014-0069#.U-takxAQcvk" target="_blank" >http://www.nrcresearchpress.com/doi/abs/10.1139/cjpp-2014-0069#.U-takxAQcvk</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1139/cjpp-2014-0069" target="_blank" >10.1139/cjpp-2014-0069</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats
Popis výsledku v původním jazyce
Epigallocatechin gallate (EGCG) is an antioxidant found in green tea. In this study, male Wistar rats were subjected either to partial hepatectomy (PHx), or a sham operation (LAP). Twenty-four hours after surgery, hepatocytes were isolated and treated with various concentrations of EGCG for up to 72 h. We then measured markers of cell viability, oxidative stress, DNA synthesis, and caspase activity. Morphological criteria, cell viability tests, and albumin synthesis revealed toxicity starting at 10 ?mol/L. DNA synthesis was higher in hepatocytes isolated from rats after PHx and inhibited by EGCG. Furthermore, EGCG increased the activity of caspases 3 and 7, seen more in hepatocytes from PHx rats. In conclusion, EGCG at a concentration of 10 ?mol/L wastoxic for hepatocytes isolated from both PHx and LAP rats.
Název v anglickém jazyce
The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats
Popis výsledku anglicky
Epigallocatechin gallate (EGCG) is an antioxidant found in green tea. In this study, male Wistar rats were subjected either to partial hepatectomy (PHx), or a sham operation (LAP). Twenty-four hours after surgery, hepatocytes were isolated and treated with various concentrations of EGCG for up to 72 h. We then measured markers of cell viability, oxidative stress, DNA synthesis, and caspase activity. Morphological criteria, cell viability tests, and albumin synthesis revealed toxicity starting at 10 ?mol/L. DNA synthesis was higher in hepatocytes isolated from rats after PHx and inhibited by EGCG. Furthermore, EGCG increased the activity of caspases 3 and 7, seen more in hepatocytes from PHx rats. In conclusion, EGCG at a concentration of 10 ?mol/L wastoxic for hepatocytes isolated from both PHx and LAP rats.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
ED - Fyziologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Canadian Journal of Physiology and Pharmacology
ISSN
0008-4212
e-ISSN
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Svazek periodika
92
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
CA - Kanada
Počet stran výsledku
6
Strana od-do
512-517
Kód UT WoS článku
000338712700013
EID výsledku v databázi Scopus
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