Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F14%3A10281583" target="_blank" >RIV/00216208:11160/14:10281583 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0018/acph-2014-0018.xml" target="_blank" >http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0018/acph-2014-0018.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acph-2014-0018" target="_blank" >10.2478/acph-2014-0018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro

Popis výsledku v původním jazyce

Catechins may influence both desirable and undesirable effects of many drugs. In this study, the in vitro effect of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate, and (-)-epigallocatechin gallate (EGCG) on the efficacy of anticancer drug doxorubicin (DOX) was studied in HCT-8 cancer cells. Rat hepatocytes were used to study the influence of EGCG on DOX hepatotoxicity. Cell proliferation and viability were studied by 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide and neutral red uptake test assays. Formation of reactive oxygen species (ROS) was determined using the dichlorofluorescein assay. All of the studied catechins (1-25 mu mol L-1) had no effect on the proliferation of intestinal cancer cells and didnot affect the antiproliferative effect of DOX (1-8 mu mol L-1) in these cells. Moreover, EGCG at 25 mu mol L-1 increased the viability of isolated hepatocytes and significantly protected these cells against DOX-induced toxicity and ROS p

Název v anglickém jazyce

Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro

Popis výsledku anglicky

Catechins may influence both desirable and undesirable effects of many drugs. In this study, the in vitro effect of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate, and (-)-epigallocatechin gallate (EGCG) on the efficacy of anticancer drug doxorubicin (DOX) was studied in HCT-8 cancer cells. Rat hepatocytes were used to study the influence of EGCG on DOX hepatotoxicity. Cell proliferation and viability were studied by 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide and neutral red uptake test assays. Formation of reactive oxygen species (ROS) was determined using the dichlorofluorescein assay. All of the studied catechins (1-25 mu mol L-1) had no effect on the proliferation of intestinal cancer cells and didnot affect the antiproliferative effect of DOX (1-8 mu mol L-1) in these cells. Moreover, EGCG at 25 mu mol L-1 increased the viability of isolated hepatocytes and significantly protected these cells against DOX-induced toxicity and ROS p

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Pharmaceutica

ISSN

1330-0075

e-ISSN

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Svazek periodika

64

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CR - Kostarická republika

Počet stran výsledku

11

Strana od-do

199-209

Kód UT WoS článku

000337705100005

EID výsledku v databázi Scopus

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