Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F12%3A43867001" target="_blank" >RIV/62690094:18470/12:43867001 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.aca.2011.12.068" target="_blank" >http://dx.doi.org/10.1016/j.aca.2011.12.068</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2011.12.068" target="_blank" >10.1016/j.aca.2011.12.068</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols

Popis výsledku v původním jazyce

Two-photon excitation enabled for the first time the observation and measurement of excited state fluorescence lifetimes from three flavanols ( ~1.0 ns for catechin and epicatechin, but {45 ps for epigallocatechin gallate (EGCG)). The shorter lifetime for EGCG is in line with a lower fluorescence quantum yield of 0.003 compared to catechin (0.015) and epicatechin (0.018). In vivo experiments with onion cells demonstrated that tryptophan and quercetin, which tend to be major contributors of background fluorescence in plant cells, have sufficiently low cross sections for two-photon excitation at 630 nm and therefore do not interfere with detection of externally added or endogenous flavanols in Allium cepa or Taxus baccata cells. Applying two-photon excitation to flavanols enabled 3-D fluorescence lifetime imaging microscopy and showed that added EGCG penetrated the whole nucleus of onion cells. Interestingly, EGCG and catechin showed different lifetime behaviour when bound to the nucleus

Název v anglickém jazyce

Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols

Popis výsledku anglicky

Two-photon excitation enabled for the first time the observation and measurement of excited state fluorescence lifetimes from three flavanols ( ~1.0 ns for catechin and epicatechin, but {45 ps for epigallocatechin gallate (EGCG)). The shorter lifetime for EGCG is in line with a lower fluorescence quantum yield of 0.003 compared to catechin (0.015) and epicatechin (0.018). In vivo experiments with onion cells demonstrated that tryptophan and quercetin, which tend to be major contributors of background fluorescence in plant cells, have sufficiently low cross sections for two-photon excitation at 630 nm and therefore do not interfere with detection of externally added or endogenous flavanols in Allium cepa or Taxus baccata cells. Applying two-photon excitation to flavanols enabled 3-D fluorescence lifetime imaging microscopy and showed that added EGCG penetrated the whole nucleus of onion cells. Interestingly, EGCG and catechin showed different lifetime behaviour when bound to the nucleus

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica chimica acta

ISSN

0003-2670

e-ISSN

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Svazek periodika

719

Číslo periodika v rámci svazku

únor 2012

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

68-75

Kód UT WoS článku

000302277900010

EID výsledku v databázi Scopus

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