Low zinc environment induces stress signaling, senescence and mixed cell death modalities in colon cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F15%3A10306938" target="_blank" >RIV/00216208:11150/15:10306938 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s10495-015-1182-5" target="_blank" >http://link.springer.com/article/10.1007/s10495-015-1182-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10495-015-1182-5" target="_blank" >10.1007/s10495-015-1182-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Low zinc environment induces stress signaling, senescence and mixed cell death modalities in colon cancer cells

Popis výsledku v původním jazyce

Currently it is not clear what type of the final cellular response (i.e. cell death modality or senescence) is induced upon chronic intracellular zinc depletion in colon cancer cells. To address this question, isogenic colon cancer lines SW480 and SW620exposed to low zinc environment were studied over the period of 6 weeks. Low zinc environment reduced total as well as free intracellular zinc content in both cell lines. Decreased intracellular zinc content resulted in changes in cellular proliferation,cell cycle distribution and activation of stress signaling. In addition, colonocytes with low zinc content displayed increased levels of oxidative stress, changes in mitochondrial activity but in the absence of significant DNA damage. Towards the end oftreatment (4th-6th week), exposed cells started to change morphologically, and typical markers of senescence as well as cell death appeared. Of two examined colon cancer cell lines, SW480 cells proved to activate predominantly senescent

Název v anglickém jazyce

Low zinc environment induces stress signaling, senescence and mixed cell death modalities in colon cancer cells

Popis výsledku anglicky

Currently it is not clear what type of the final cellular response (i.e. cell death modality or senescence) is induced upon chronic intracellular zinc depletion in colon cancer cells. To address this question, isogenic colon cancer lines SW480 and SW620exposed to low zinc environment were studied over the period of 6 weeks. Low zinc environment reduced total as well as free intracellular zinc content in both cell lines. Decreased intracellular zinc content resulted in changes in cellular proliferation,cell cycle distribution and activation of stress signaling. In addition, colonocytes with low zinc content displayed increased levels of oxidative stress, changes in mitochondrial activity but in the absence of significant DNA damage. Towards the end oftreatment (4th-6th week), exposed cells started to change morphologically, and typical markers of senescence as well as cell death appeared. Of two examined colon cancer cell lines, SW480 cells proved to activate predominantly senescent

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Apoptosis : an international journal on programmed cell death

ISSN

1360-8185

e-ISSN

—

Svazek periodika

20

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

1651-1665

Kód UT WoS článku

000362881900009

EID výsledku v databázi Scopus

2-s2.0-84943798316