Flubendazole induces mitotic catastrophe and senescence in colon cancer cells in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F16%3A10322146" target="_blank" >RIV/00216208:11150/16:10322146 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/16:10322146

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/jphp.12503/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jphp.12503/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jphp.12503" target="_blank" >10.1111/jphp.12503</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flubendazole induces mitotic catastrophe and senescence in colon cancer cells in vitro

Popis výsledku v původním jazyce

Objectives: Flubendazole (FLU), a member of benzimidazole family of anthelmintic drugs, is able to inhibit proliferation of various cancer cells. The aim of present study was to elucidate the mechanisms of antiproliferative effect of FLU on colorectal cancer cells in vitro. Methods: The effect of FLU on proliferation, microtubular network, DNA content, caspase activation and senescence induction was studied in SW480 and SW620 cell lines. Key findings: Flubendazole significantly affected cell proliferation in a pattern typical for mitotic inhibitor. This was accompanied by decrease in cyclin D1 levels, increase in cyclin B1 levels, activation of caspase 2 and caspase 3/7 and PARP cleavage. Morphological observations revealed disruption of microtubular network, irregular mitotic spindles, formation of giant multinucleated cells and increase in nuclear area and DNA content. In SW620 cell line, 37.5% giant multinucleated cells induced by FLU treatment showed positivity for SA-beta-galactosidase staining. Cell lines were able to recover from the treatment and this process was faster in SW480 cells. Conclusion: Flubendazole in low concentration temporarily inhibits cell proliferation and induces mitotic catastrophe and premature senescence in human colon cancer cells in vitro.

Název v anglickém jazyce

Flubendazole induces mitotic catastrophe and senescence in colon cancer cells in vitro

Popis výsledku anglicky

Objectives: Flubendazole (FLU), a member of benzimidazole family of anthelmintic drugs, is able to inhibit proliferation of various cancer cells. The aim of present study was to elucidate the mechanisms of antiproliferative effect of FLU on colorectal cancer cells in vitro. Methods: The effect of FLU on proliferation, microtubular network, DNA content, caspase activation and senescence induction was studied in SW480 and SW620 cell lines. Key findings: Flubendazole significantly affected cell proliferation in a pattern typical for mitotic inhibitor. This was accompanied by decrease in cyclin D1 levels, increase in cyclin B1 levels, activation of caspase 2 and caspase 3/7 and PARP cleavage. Morphological observations revealed disruption of microtubular network, irregular mitotic spindles, formation of giant multinucleated cells and increase in nuclear area and DNA content. In SW620 cell line, 37.5% giant multinucleated cells induced by FLU treatment showed positivity for SA-beta-galactosidase staining. Cell lines were able to recover from the treatment and this process was faster in SW480 cells. Conclusion: Flubendazole in low concentration temporarily inhibits cell proliferation and induces mitotic catastrophe and premature senescence in human colon cancer cells in vitro.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Pharmacy and Pharmacology

ISSN

0022-3573

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

208-218

Kód UT WoS článku

000371246600007

EID výsledku v databázi Scopus

2-s2.0-84958768631