Decellularized Skeletal Muscle: A Promising Biologic Scaffold for Tissue Engineering
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F17%3A10368705" target="_blank" >RIV/00216208:11150/17:10368705 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1166/jbt.2017.1588" target="_blank" >http://dx.doi.org/10.1166/jbt.2017.1588</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1166/jbt.2017.1588" target="_blank" >10.1166/jbt.2017.1588</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Decellularized Skeletal Muscle: A Promising Biologic Scaffold for Tissue Engineering
Popis výsledku v původním jazyce
Stroma of mammalian organs including skeletal muscle is so complex in its macroscopic structure, microarchitecture and molecular composition that its detailed artificial reconstruction is out of reach. Nevertheless a removal of cells from the organ can be used to yield the extracellular matrix (ECM) mimicking many features of the original tissue complexity. We decellularized the skeletal muscle using combination of the osmotic shock, chemical and DNase treatment and obtained the bioscaffolds free of cellular and cytoplasmic components. Moreover, the scaffold ECM reflected light microscopic organization of the muscle coverings, blood vessels and peripheral nerves and also retained 3D organization of basal laminas and collagen fibrils as confirmed by transmission electron microscopy. Laminin and collagen type IV remained organized within basal laminas supporting cell attachment and navigation. Recellularization of the scaffolds with C2C12 cells in vitro confirmed cytocompatibility of the decellularized ECM as well as its ability to support basic physiological cellular functions as the myoblasts were aligned with ECM microstructure. After 12 days in vitro the samples did not show any signs of abnormalities, regression or cell death. These data strongly indicated that skeletal muscle decellularization could be considered as a promising approach in construction of bioscaffolds suitable for muscle tissue replacement.
Název v anglickém jazyce
Decellularized Skeletal Muscle: A Promising Biologic Scaffold for Tissue Engineering
Popis výsledku anglicky
Stroma of mammalian organs including skeletal muscle is so complex in its macroscopic structure, microarchitecture and molecular composition that its detailed artificial reconstruction is out of reach. Nevertheless a removal of cells from the organ can be used to yield the extracellular matrix (ECM) mimicking many features of the original tissue complexity. We decellularized the skeletal muscle using combination of the osmotic shock, chemical and DNase treatment and obtained the bioscaffolds free of cellular and cytoplasmic components. Moreover, the scaffold ECM reflected light microscopic organization of the muscle coverings, blood vessels and peripheral nerves and also retained 3D organization of basal laminas and collagen fibrils as confirmed by transmission electron microscopy. Laminin and collagen type IV remained organized within basal laminas supporting cell attachment and navigation. Recellularization of the scaffolds with C2C12 cells in vitro confirmed cytocompatibility of the decellularized ECM as well as its ability to support basic physiological cellular functions as the myoblasts were aligned with ECM microstructure. After 12 days in vitro the samples did not show any signs of abnormalities, regression or cell death. These data strongly indicated that skeletal muscle decellularization could be considered as a promising approach in construction of bioscaffolds suitable for muscle tissue replacement.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10601 - Cell biology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA15-09161S" target="_blank" >GA15-09161S: Remodelace svalu na podkladě extracelulární matrix osázené funkčně charakterizovanými buňkami.</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal Of Biomaterials And Tissue Engineering
ISSN
2157-9083
e-ISSN
—
Svazek periodika
7
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
8
Strana od-do
491-498
Kód UT WoS článku
000398930300008
EID výsledku v databázi Scopus
2-s2.0-85015615042