NANOSTRUCTURE AND BIOACTIVITY OF MOUSE LUNG EXTRACELLULAR MATRIX SCAFFOLDS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00100758" target="_blank" >RIV/00216224:14110/18:00100758 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/18:00070389

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

NANOSTRUCTURE AND BIOACTIVITY OF MOUSE LUNG EXTRACELLULAR MATRIX SCAFFOLDS

Popis výsledku v původním jazyce

Extracellular matrix (ECM) forms an essential part of tissue microenvironment. Compositional and structural properties of ECM modulate behaviour of cells, including their differentiation, proliferation, and turnover. Here we aimed at detailed characterization of the nanostructure of lung ECM and evaluation of its bioactivity on cells grown in vitro. Mouse lungs were decellularized using 0.2 % sodium dodecyl sulphate, hypotonic solutions, and DNase. Morphological analysis of the resulting ECM scaffolds was performed by means of transmission and scanning electron microscopy. The ECM scaffolds retained 3D architecture of the main lung anatomical regions: the alveolar region and the blood/ airway network. The region-specific ECM nanotopology and organization of ECM macromolecules such as fibres of collagen, elastin, and fibrillin were characterized. The lung ECM scaffolds were also homogenized and applied as a supplement to growth medium on primary lung cells in vitro to test the bioactivity of lung ECM. We demonstrate that homogenized ECM does not have a negative impact on the proliferation rate of primary lung cells. In conclusion, we herein show at nanoscale the morphological characteristics of lung ECM and demonstrate that lung ECM produced by decellularization procedure is compatible with in vitro cultured lung cells. These findings add to a better understanding of cells with their natural environment and can be valuable when designing applications of ECM for purposes in tissue engineering.

Název v anglickém jazyce

NANOSTRUCTURE AND BIOACTIVITY OF MOUSE LUNG EXTRACELLULAR MATRIX SCAFFOLDS

Popis výsledku anglicky

Extracellular matrix (ECM) forms an essential part of tissue microenvironment. Compositional and structural properties of ECM modulate behaviour of cells, including their differentiation, proliferation, and turnover. Here we aimed at detailed characterization of the nanostructure of lung ECM and evaluation of its bioactivity on cells grown in vitro. Mouse lungs were decellularized using 0.2 % sodium dodecyl sulphate, hypotonic solutions, and DNase. Morphological analysis of the resulting ECM scaffolds was performed by means of transmission and scanning electron microscopy. The ECM scaffolds retained 3D architecture of the main lung anatomical regions: the alveolar region and the blood/ airway network. The region-specific ECM nanotopology and organization of ECM macromolecules such as fibres of collagen, elastin, and fibrillin were characterized. The lung ECM scaffolds were also homogenized and applied as a supplement to growth medium on primary lung cells in vitro to test the bioactivity of lung ECM. We demonstrate that homogenized ECM does not have a negative impact on the proliferation rate of primary lung cells. In conclusion, we herein show at nanoscale the morphological characteristics of lung ECM and demonstrate that lung ECM produced by decellularization procedure is compatible with in vitro cultured lung cells. These findings add to a better understanding of cells with their natural environment and can be valuable when designing applications of ECM for purposes in tissue engineering.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH &amp; APPLICATION (NANOCON 2017)

ISBN

9788087294819

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

560-566

Název nakladatele

TANGER LTD

Místo vydání

SLEZSKA

Místo konání akce

Brno, CZECH REPUBLIC

Datum konání akce

18. 10. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000452823300092