Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F24%3A00136048" target="_blank" >RIV/00216224:14110/24:00136048 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-3674-9_3" target="_blank" >10.1007/978-1-0716-3674-9_3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering

Popis výsledku v původním jazyce

Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting.

Název v anglickém jazyce

Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering

Popis výsledku anglicky

Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/NV16-31501A" target="_blank" >NV16-31501A: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

3D Cell Culture. Methods in Molecular Biology

ISBN

9781071636732

Počet stran výsledku

13

Strana od-do

21-33

Počet stran knihy

336

Název nakladatele

Humana

Místo vydání

New York

Kód UT WoS kapitoly

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