Restriction Factor Expression in Vertically Infected Children Living With HIV-1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F21%3A10439019" target="_blank" >RIV/00216208:11150/21:10439019 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=QNl.LGiqny" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=QNl.LGiqny</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1097/INF.0000000000002924" target="_blank" >10.1097/INF.0000000000002924</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Restriction Factor Expression in Vertically Infected Children Living With HIV-1

Popis výsledku v původním jazyce

Introduction: Around 1.7 million children are estimated to live with HIV-1 worldwide, and about 160,000 infants are newly infected every year. Since adaptive immunity takes time to mature and develop in infants, and maternal antibodies provide limited antiviral activity, innate and intrinsic immunity against HIV-1 in the young is of critical importance. Intrinsic restriction factors are cellular proteins that effectively inhibit HIV-1 replication in vitro, but there is limited understanding of their role in vivo, and little to no data has been reported on the expression of host restriction factors in children. We hypothesized that restriction factor expression might be particularly important in children living with HIV-1 and correlate with disease progression. Methods: We analyzed gene expression of APOBEC3A, APOBEC3C, APOBEC3G, APOBEC3H, SAMHD1, ISG15, CDKN1A, MX2, TRIM5, and SLFN11 by qPCR in 121 samples of CD4+ T cells from vertically infected children living with HIV-1. Cell surface expression of BST-2/tetherin and markers of CD4+ T-cell activation were analyzed by flow cytometry. Results: After adjusting for gender and age, BST-2/tetherin expression on CD4+ T cells showed significant positive correlation with viral load (P=0.0006; rho = 0.33), CD4+ T-cell activation (P &lt; 0.0001; rho = 0.53), CD8+ T-cell activation (P &lt; 0.0001;. = 0.53), and a negative correlation with CD4+ T-cell counts (P = 0.0008; rho = -0.33). The expression of SAMHD1 correlated negatively with markers of T-cell activation (P = 0.046; rho = -0.22). Discussion: These results suggest an important role of some restriction factors in the pathogenesis of HIV-1 in children.

Název v anglickém jazyce

Restriction Factor Expression in Vertically Infected Children Living With HIV-1

Popis výsledku anglicky

Introduction: Around 1.7 million children are estimated to live with HIV-1 worldwide, and about 160,000 infants are newly infected every year. Since adaptive immunity takes time to mature and develop in infants, and maternal antibodies provide limited antiviral activity, innate and intrinsic immunity against HIV-1 in the young is of critical importance. Intrinsic restriction factors are cellular proteins that effectively inhibit HIV-1 replication in vitro, but there is limited understanding of their role in vivo, and little to no data has been reported on the expression of host restriction factors in children. We hypothesized that restriction factor expression might be particularly important in children living with HIV-1 and correlate with disease progression. Methods: We analyzed gene expression of APOBEC3A, APOBEC3C, APOBEC3G, APOBEC3H, SAMHD1, ISG15, CDKN1A, MX2, TRIM5, and SLFN11 by qPCR in 121 samples of CD4+ T cells from vertically infected children living with HIV-1. Cell surface expression of BST-2/tetherin and markers of CD4+ T-cell activation were analyzed by flow cytometry. Results: After adjusting for gender and age, BST-2/tetherin expression on CD4+ T cells showed significant positive correlation with viral load (P=0.0006; rho = 0.33), CD4+ T-cell activation (P &lt; 0.0001; rho = 0.53), CD8+ T-cell activation (P &lt; 0.0001;. = 0.53), and a negative correlation with CD4+ T-cell counts (P = 0.0008; rho = -0.33). The expression of SAMHD1 correlated negatively with markers of T-cell activation (P = 0.046; rho = -0.22). Discussion: These results suggest an important role of some restriction factors in the pathogenesis of HIV-1 in children.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30303 - Infectious Diseases

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pediatric Infectious Disease Journal

ISSN

0891-3668

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

144-146

Kód UT WoS článku

000639298700025

EID výsledku v databázi Scopus

2-s2.0-85100070395