Circumventing the requirement of binding saturation for receptor quantification using interaction kinetic extrapolation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F11%3A10118908" target="_blank" >RIV/00216208:11160/11:10118908 - isvavai.cz</a>

Výsledek na webu

<a href="http://ovidsp.tx.ovid.com/sp-3.5.1a/ovidweb.cgi?T=JS&PAGE=fulltext&D=ovft&AN=00006231-201109000-00015&NEWS=N" target="_blank" >http://ovidsp.tx.ovid.com/sp-3.5.1a/ovidweb.cgi?T=JS&PAGE=fulltext&D=ovft&AN=00006231-201109000-00015&NEWS=N</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1097/MNM.0b013e3283483e1c" target="_blank" >10.1097/MNM.0b013e3283483e1c</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Circumventing the requirement of binding saturation for receptor quantification using interaction kinetic extrapolation

Popis výsledku v původním jazyce

Quantification of the number of receptors per cell (NRPC) is important when assessing whether a tumor surface biomarker is suitable for medical imaging. One common method for NPRC quantification is to use a binding saturation assay, which is time consuming and requires large amounts of reagents. The aim of this study was to evaluate an alternative method based on kinetic extrapolation (KEX) and compare it with the classical manual saturation technique with regard to accuracy as well as time and reagentconsumption. From the observations described in this study, we believe that the KEX method enables fast, credible, and easy NRPC quantification with a reduction in reagent consumption.

Název v anglickém jazyce

Circumventing the requirement of binding saturation for receptor quantification using interaction kinetic extrapolation

Popis výsledku anglicky

Quantification of the number of receptors per cell (NRPC) is important when assessing whether a tumor surface biomarker is suitable for medical imaging. One common method for NPRC quantification is to use a binding saturation assay, which is time consuming and requires large amounts of reagents. The aim of this study was to evaluate an alternative method based on kinetic extrapolation (KEX) and compare it with the classical manual saturation technique with regard to accuracy as well as time and reagentconsumption. From the observations described in this study, we believe that the KEX method enables fast, credible, and easy NRPC quantification with a reduction in reagent consumption.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nuclear Medicine Communications

ISSN

0143-3636

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

863-867

Kód UT WoS článku

000294345300015

EID výsledku v databázi Scopus

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