A comparison of in vitro methods for determining the membrane receptor expression in cell lines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124799" target="_blank" >RIV/00216208:11160/12:10124799 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0969805112000492" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0969805112000492</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.nucmedbio.2012.02.007" target="_blank" >10.1016/j.nucmedbio.2012.02.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A comparison of in vitro methods for determining the membrane receptor expression in cell lines

Popis výsledku v původním jazyce

Introduction: Determining the number of expressed receptors per cell (NRPC) in cell lines is an important prerequisite for many experimental procedures in biomedical research. This paper focuses on the comparison of a newly developed method of determining NRPC - the Kinetic extrapolation method (KEX) - with the standard saturation method. These two methods, both based on radiolabeled ligand-receptor binding, were compared with the data on receptor expression found using quantified western blotting. Methods: Four cell lines with different expressions of epidermal growth factor receptor (EGFR) were chosen for the experiment: A431, HaCaT, HCT116 and HepG2. Two radiolabeled monoclonal antibodies specific for EGFR were used as ligands: [I-131]-cetuximab and[I-131]-panitumumab. The classic manual technique based on the saturation of cell receptors was performed on cells seeded in 24-well plates. kThe KEX method uses the LigandTracer, a special instrument which detects ligand retention in re

Název v anglickém jazyce

A comparison of in vitro methods for determining the membrane receptor expression in cell lines

Popis výsledku anglicky

Introduction: Determining the number of expressed receptors per cell (NRPC) in cell lines is an important prerequisite for many experimental procedures in biomedical research. This paper focuses on the comparison of a newly developed method of determining NRPC - the Kinetic extrapolation method (KEX) - with the standard saturation method. These two methods, both based on radiolabeled ligand-receptor binding, were compared with the data on receptor expression found using quantified western blotting. Methods: Four cell lines with different expressions of epidermal growth factor receptor (EGFR) were chosen for the experiment: A431, HaCaT, HCT116 and HepG2. Two radiolabeled monoclonal antibodies specific for EGFR were used as ligands: [I-131]-cetuximab and[I-131]-panitumumab. The classic manual technique based on the saturation of cell receptors was performed on cells seeded in 24-well plates. kThe KEX method uses the LigandTracer, a special instrument which detects ligand retention in re

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP304%2F10%2F1738" target="_blank" >GAP304/10/1738: Radioaktivně značené neuropeptidy cílené pro diagnostiku a terapii nádorů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nuclear Medicine and Biology

ISSN

0969-8051

e-ISSN

—

Svazek periodika

39

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

893-896

Kód UT WoS článku

000309033800001

EID výsledku v databázi Scopus

—