Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10145719" target="_blank" >RIV/00216208:11160/13:10145719 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0039914012008673" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0039914012008673</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2012.10.036" target="_blank" >10.1016/j.talanta.2012.10.036</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids

Popis výsledku v původním jazyce

This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of this work was to extend the chromatographic capabilities of the SIC system. Despite the particle-packed columns reaching system pressures of {= 610 PSI, their conditions matched those of a commercially produced and optimised SIC system. The selectivity of each of the tested columns, Ascentis (R) Express RP-Amide, Ascentis (R) Express Phenyl-Hexyl and Ascentis (R) Express C18 (30 mm x 4.6 mm, core-shell particle size 2.7 mu m), was compared by their ability to separate seven phenolic acids that are secondary metabolite substances widely distributed in plants. The separations of all of the components were performed by isocratic elution using binary mobile phases composed of acetonitrile and 0.065% phosphoric acid at pH 2.4 (a specific ratio was used for each column) at a flow-rate of 0.60 mL/min. The volume of the mobile phase was 3

Název v anglickém jazyce

Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids

Popis výsledku anglicky

This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of this work was to extend the chromatographic capabilities of the SIC system. Despite the particle-packed columns reaching system pressures of {= 610 PSI, their conditions matched those of a commercially produced and optimised SIC system. The selectivity of each of the tested columns, Ascentis (R) Express RP-Amide, Ascentis (R) Express Phenyl-Hexyl and Ascentis (R) Express C18 (30 mm x 4.6 mm, core-shell particle size 2.7 mu m), was compared by their ability to separate seven phenolic acids that are secondary metabolite substances widely distributed in plants. The separations of all of the components were performed by isocratic elution using binary mobile phases composed of acetonitrile and 0.065% phosphoric acid at pH 2.4 (a specific ratio was used for each column) at a flow-rate of 0.60 mL/min. The volume of the mobile phase was 3

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

103

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

221-227

Kód UT WoS článku

000314010600032

EID výsledku v databázi Scopus

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