Construction and characterization of peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1 alpha over-expressing cell line derived from human hepatocyte carcinoma HepG2 cells)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10146004" target="_blank" >RIV/00216208:11160/13:10146004 - isvavai.cz</a>

Výsledek na webu

<a href="http://biomed.papers.upol.cz/pdfs/bio/2013/03/02.pdf" target="_blank" >http://biomed.papers.upol.cz/pdfs/bio/2013/03/02.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2012.075" target="_blank" >10.5507/bp.2012.075</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Construction and characterization of peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1 alpha over-expressing cell line derived from human hepatocyte carcinoma HepG2 cells)

Popis výsledku v původním jazyce

Aims. The aim was develop stable human cell line stable over-expressing transcription co-activator peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC-1 alpha) with restored hepatospecific functions and increased expression of major xenobiotic metabolizing enzymes. Methods. Six clones of HepG2-PGC-1 alpha and one control clone HepG2-pcDNA3 were isolated and analyzed for secretion of hepatospecific markers, fibrinogen, albumin and alpha1-antitrypsin. Expression levels of protein and mRNA of hepatocyte nuclear factor (HNF4 alpha), pregnane X receptor (PXR) and aryl hydrocarbon receptor (AhR) were determined. We measured basal and ligand inducible expression of CYP1A1 and CYP3A4. Results. Stably transfected cell line HepG2-PGC-1 alpha derived from HepG2 cells over-expressing PGC-1 alpha displayed increased secretion of fibrinogen, but not albumin or alpha1-antitrypsin compared to parent HepG2 cells. We found increased levels of HNF4 alpha, PXR and AhR proteins but n

Název v anglickém jazyce

Construction and characterization of peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1 alpha over-expressing cell line derived from human hepatocyte carcinoma HepG2 cells)

Popis výsledku anglicky

Aims. The aim was develop stable human cell line stable over-expressing transcription co-activator peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC-1 alpha) with restored hepatospecific functions and increased expression of major xenobiotic metabolizing enzymes. Methods. Six clones of HepG2-PGC-1 alpha and one control clone HepG2-pcDNA3 were isolated and analyzed for secretion of hepatospecific markers, fibrinogen, albumin and alpha1-antitrypsin. Expression levels of protein and mRNA of hepatocyte nuclear factor (HNF4 alpha), pregnane X receptor (PXR) and aryl hydrocarbon receptor (AhR) were determined. We measured basal and ligand inducible expression of CYP1A1 and CYP3A4. Results. Stably transfected cell line HepG2-PGC-1 alpha derived from HepG2 cells over-expressing PGC-1 alpha displayed increased secretion of fibrinogen, but not albumin or alpha1-antitrypsin compared to parent HepG2 cells. We found increased levels of HNF4 alpha, PXR and AhR proteins but n

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia

ISSN

1213-8118

e-ISSN

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Svazek periodika

157

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

214-221

Kód UT WoS článku

000329091100003

EID výsledku v databázi Scopus

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