A fast on-line micro-column sample pretreatment with a HPLC column switching technique and large volume sample injection for determination of non-steroidal anti-inflammatory drugs in human serum
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10146042" target="_blank" >RIV/00216208:11160/13:10146042 - isvavai.cz</a>
Výsledek na webu
<a href="http://pubs.rsc.org/en/content/articlehtml/2013/ay/c3ay40505b" target="_blank" >http://pubs.rsc.org/en/content/articlehtml/2013/ay/c3ay40505b</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c3ay40505b" target="_blank" >10.1039/c3ay40505b</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A fast on-line micro-column sample pretreatment with a HPLC column switching technique and large volume sample injection for determination of non-steroidal anti-inflammatory drugs in human serum
Popis výsledku v původním jazyce
A fully automated HPLC column-switching method with fast sample pretreatment on a short micro-column was developed for sensitive and quantitative determination of non-steroidal anti-inflammatory drugs (ketoprofen, ibuprofen, sodium diclofenac and flurbiprofen) in human serum with UV detection. The serum samples were diluted 3 : 1, with 2% acetic acid in acetonitrile, and centrifuged at 4200g for 10 min. 200 mL of supernatant was injected directly into the HPLC system. An on-line sample clean-up was achieved on a pretreatment micro-column LiChrospher (R) RP-18 ADS 25 m-2.0 mm, 25 mm (Merck) with a washing mobile phase (acetonitrile : 0.085% phosphoric acid; 10 : 90) at a flow rate of 2.5 mu L min(-1). The valve switch to an analytical column was set atthe 7th min in a back-flush mode. Separation of all antiflogistics and internal standard butylparaben was performed using a Chromolith Performance RP 18 100-4.6 mm column (Merck) and a mobile phase consisting of 0.085% phosphoric acid and
Název v anglickém jazyce
A fast on-line micro-column sample pretreatment with a HPLC column switching technique and large volume sample injection for determination of non-steroidal anti-inflammatory drugs in human serum
Popis výsledku anglicky
A fully automated HPLC column-switching method with fast sample pretreatment on a short micro-column was developed for sensitive and quantitative determination of non-steroidal anti-inflammatory drugs (ketoprofen, ibuprofen, sodium diclofenac and flurbiprofen) in human serum with UV detection. The serum samples were diluted 3 : 1, with 2% acetic acid in acetonitrile, and centrifuged at 4200g for 10 min. 200 mL of supernatant was injected directly into the HPLC system. An on-line sample clean-up was achieved on a pretreatment micro-column LiChrospher (R) RP-18 ADS 25 m-2.0 mm, 25 mm (Merck) with a washing mobile phase (acetonitrile : 0.085% phosphoric acid; 10 : 90) at a flow rate of 2.5 mu L min(-1). The valve switch to an analytical column was set atthe 7th min in a back-flush mode. Separation of all antiflogistics and internal standard butylparaben was performed using a Chromolith Performance RP 18 100-4.6 mm column (Merck) and a mobile phase consisting of 0.085% phosphoric acid and
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Analytical Methods
ISSN
1759-9660
e-ISSN
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Svazek periodika
5
Číslo periodika v rámci svazku
21
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
6153-6159
Kód UT WoS článku
000325722600044
EID výsledku v databázi Scopus
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