A rapid HPLC column switching method for sample preparation and determination of beta-carotene in food supplements
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10146037" target="_blank" >RIV/00216208:11160/13:10146037 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0308814613005098" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0308814613005098</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.foodchem.2013.04.063" target="_blank" >10.1016/j.foodchem.2013.04.063</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A rapid HPLC column switching method for sample preparation and determination of beta-carotene in food supplements
Popis výsledku v původním jazyce
A simple and automated HPLC column-switching method with rapid sample pretreatment has been developed for quantitative determination of beta-carotene in food supplements. Commercially samples of food supplements were dissolved in chloroform with help ofsaponification with 1 M solution of sodium hydroxide in ultrasound bath. A 20-min sample dissolution/extraction step was necessary before chromatography analysis to transfer beta-carotene from solid state of food supplements preparations (capsules, tablets) to chloroform solution. Sample volume - 3 mu L of chloroform phase was directly injected into the HPLC system. Next on-line sample clean-up was achieved on the pretreatment precolumn Chromolith Guard Cartridge RP-18e (Merck), 10 x 4.6 mm, with a washing mobile phase (methanol:water, 92:8, (v/v)) at a flow rate of 1.5 mL/min. Valve switch to analytical column was set at 2.5 min in a back-flush mode. After column switching to the analytical column Ascentis Express C-18, 30 x 4.6 mm, pa
Název v anglickém jazyce
A rapid HPLC column switching method for sample preparation and determination of beta-carotene in food supplements
Popis výsledku anglicky
A simple and automated HPLC column-switching method with rapid sample pretreatment has been developed for quantitative determination of beta-carotene in food supplements. Commercially samples of food supplements were dissolved in chloroform with help ofsaponification with 1 M solution of sodium hydroxide in ultrasound bath. A 20-min sample dissolution/extraction step was necessary before chromatography analysis to transfer beta-carotene from solid state of food supplements preparations (capsules, tablets) to chloroform solution. Sample volume - 3 mu L of chloroform phase was directly injected into the HPLC system. Next on-line sample clean-up was achieved on the pretreatment precolumn Chromolith Guard Cartridge RP-18e (Merck), 10 x 4.6 mm, with a washing mobile phase (methanol:water, 92:8, (v/v)) at a flow rate of 1.5 mL/min. Valve switch to analytical column was set at 2.5 min in a back-flush mode. After column switching to the analytical column Ascentis Express C-18, 30 x 4.6 mm, pa
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Food Chemistry
ISSN
0308-8146
e-ISSN
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Svazek periodika
141
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
5
Strana od-do
1433-1437
Kód UT WoS článku
000321803500105
EID výsledku v databázi Scopus
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