Modulatory Effects of Quercetin and Rutin on the Activity, Expression and Inducibility of CYP1A1 in Intestinal HCT-8 Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10189501" target="_blank" >RIV/00216208:11160/13:10189501 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/ptr.4992/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/ptr.4992/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ptr.4992" target="_blank" >10.1002/ptr.4992</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Modulatory Effects of Quercetin and Rutin on the Activity, Expression and Inducibility of CYP1A1 in Intestinal HCT-8 Cells

Popis výsledku v původním jazyce

Flavonoids, plant secondary metabolites present in fruits and vegetables, show antioxidant and anti-tumorigenic effects in vitro, but their poor absorption from gastrointestinal tract limits their systemic efficacy in humans. On the other hand, flavonoids could protect intestinal cells against carcinogens by their potential to inhibit the enzymes metabolizing pre-carcinogenic compounds (e.g. CYP1A) to reactive ones. This work was designed to test the effect of quercetin (the most abundant flavonoid) andrutin (the most abundant glycosidic form) on the activity, expression and inducibility of CYP1A in intestinal HCT-8 cells. CYP1A enzymatic activity was measured by ethoxyresorufin-O-deethylase (EROD) activity, CYP1A protein expression was detected by western blotting. The effect of flavonoids on viability of cells was examined by neutral red uptake test. No cytotoxic effect of flavonoids up to 50M concentration was observed. Quercetin significantly inhibited EROD activity in the cells,

Název v anglickém jazyce

Modulatory Effects of Quercetin and Rutin on the Activity, Expression and Inducibility of CYP1A1 in Intestinal HCT-8 Cells

Popis výsledku anglicky

Flavonoids, plant secondary metabolites present in fruits and vegetables, show antioxidant and anti-tumorigenic effects in vitro, but their poor absorption from gastrointestinal tract limits their systemic efficacy in humans. On the other hand, flavonoids could protect intestinal cells against carcinogens by their potential to inhibit the enzymes metabolizing pre-carcinogenic compounds (e.g. CYP1A) to reactive ones. This work was designed to test the effect of quercetin (the most abundant flavonoid) andrutin (the most abundant glycosidic form) on the activity, expression and inducibility of CYP1A in intestinal HCT-8 cells. CYP1A enzymatic activity was measured by ethoxyresorufin-O-deethylase (EROD) activity, CYP1A protein expression was detected by western blotting. The effect of flavonoids on viability of cells was examined by neutral red uptake test. No cytotoxic effect of flavonoids up to 50M concentration was observed. Quercetin significantly inhibited EROD activity in the cells,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP303%2F12%2FG163" target="_blank" >GBP303/12/G163: Centrum interakcí potravních doplňků s léčivy a nutrigenetiky</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Phytotherapy Research

ISSN

0951-418X

e-ISSN

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Svazek periodika

27

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

1889-1893

Kód UT WoS článku

000328210400023

EID výsledku v databázi Scopus

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