Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F14%3A10282192" target="_blank" >RIV/00216208:11160/14:10282192 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400020/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400020/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201400020" target="_blank" >10.1002/jssc.201400020</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography

Popis výsledku v původním jazyce

The retention behavior of a large group of analytes (35) with varied properties (pK(a) and log P) was studied on eight hydrophilic interaction LC columns with different surfaces, stationary phase chemistries, and types of particles. The acetonitrile content (5-95%), buffer concentration (0.5-200 mM), and pH of the mobile phase (3.8 and 6.8) were evaluated for their effects on the retention behavior. The type of stationary phase had a significant impact on the selectivity and retention time of the testedanalytes. Completely different selectivity was observed on the aminopropyl stationary phase. In this study, the influence of the buffer concentration was similar for all tested columns, except for the aminopropyl stationary phase. Increasing the bufferconcentration led to decreased retention times for the basic compounds and increased retention times for the acidic compounds, while the inverse behavior was observed on the aminopropyl stationary phase. The selectivity of the individual

Název v anglickém jazyce

Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography

Popis výsledku anglicky

The retention behavior of a large group of analytes (35) with varied properties (pK(a) and log P) was studied on eight hydrophilic interaction LC columns with different surfaces, stationary phase chemistries, and types of particles. The acetonitrile content (5-95%), buffer concentration (0.5-200 mM), and pH of the mobile phase (3.8 and 6.8) were evaluated for their effects on the retention behavior. The type of stationary phase had a significant impact on the selectivity and retention time of the testedanalytes. Completely different selectivity was observed on the aminopropyl stationary phase. In this study, the influence of the buffer concentration was similar for all tested columns, except for the aminopropyl stationary phase. Increasing the bufferconcentration led to decreased retention times for the basic compounds and increased retention times for the acidic compounds, while the inverse behavior was observed on the aminopropyl stationary phase. The selectivity of the individual

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.30.0022" target="_blank" >EE2.3.30.0022: Podpora vytváření, rozvoje a mobility kvalitních výzkumně-vývojových týmů na Univerzitě Karlově</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

1297-1307

Kód UT WoS článku

000337679600011

EID výsledku v databázi Scopus

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