Systematic evaluation of selected supercritical fluid chromatography diol- and diethylamine-based columns for application in hydrophilic interaction liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10403529" target="_blank" >RIV/00216208:11310/19:10403529 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=JL0fubJd2y" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=JL0fubJd2y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/sscp.201900023" target="_blank" >10.1002/sscp.201900023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Systematic evaluation of selected supercritical fluid chromatography diol- and diethylamine-based columns for application in hydrophilic interaction liquid chromatography

Popis výsledku v původním jazyce

Hydrophilic interaction liquid chromatography has become a useful method for separation of polar and hydrophilic analytes, i.e. compounds that are difficult to separate in reversed-phase chromatography. The retention mechanism in hydrophilic interaction liquid chromatography is rather complex. Different types of interactions contribute to retention, depending on the properties of stationary phase, mobile phase and nature of analytes. Many columns for hydrophilic interaction liquid chromatography are available and the new stationary phases are still under development. In our study systematic examination to describe two novel columns, intended for supercritical fluid chromatography, as potential stationary phases for hydrophilic interaction liquid chromatography was performed. The supercritical fluid chromatography columns contained polar diethylamine ligand and diol ligand bonded to ethylene bridged hybrid particles. The retentivity and selectivity of the stationary phases were evaluated using a variety of test compounds. The impacts of acetonitrile content, buffer pH and concentration on the retention were investigated. Distinct mixed-mode behavior of the diethylamine column was observed; significant contribution of anion-exchange interaction to retention was confirmed. The DIOL phase has neutral character and retained analytes predominantly by hydrophilic interaction liquid chromatography mechanism.

Název v anglickém jazyce

Systematic evaluation of selected supercritical fluid chromatography diol- and diethylamine-based columns for application in hydrophilic interaction liquid chromatography

Popis výsledku anglicky

Hydrophilic interaction liquid chromatography has become a useful method for separation of polar and hydrophilic analytes, i.e. compounds that are difficult to separate in reversed-phase chromatography. The retention mechanism in hydrophilic interaction liquid chromatography is rather complex. Different types of interactions contribute to retention, depending on the properties of stationary phase, mobile phase and nature of analytes. Many columns for hydrophilic interaction liquid chromatography are available and the new stationary phases are still under development. In our study systematic examination to describe two novel columns, intended for supercritical fluid chromatography, as potential stationary phases for hydrophilic interaction liquid chromatography was performed. The supercritical fluid chromatography columns contained polar diethylamine ligand and diol ligand bonded to ethylene bridged hybrid particles. The retentivity and selectivity of the stationary phases were evaluated using a variety of test compounds. The impacts of acetonitrile content, buffer pH and concentration on the retention were investigated. Distinct mixed-mode behavior of the diethylamine column was observed; significant contribution of anion-exchange interaction to retention was confirmed. The DIOL phase has neutral character and retained analytes predominantly by hydrophilic interaction liquid chromatography mechanism.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA16-05942S" target="_blank" >GA16-05942S: Gradientová chromatofokusace a izoelektrická fokusace - nástroje pro separaci proteinů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Separation Science Plus [online]

ISSN

2573-1815

e-ISSN

—

Svazek periodika

2

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

81-88

Kód UT WoS článku

000476952900002

EID výsledku v databázi Scopus

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