Effect of water on the retention on diol and amide columns in hydrophilic interaction liquid chromatography
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39911192" target="_blank" >RIV/00216275:25310/17:39911192 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1002/jssc.201601044" target="_blank" >http://dx.doi.org/10.1002/jssc.201601044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201601044" target="_blank" >10.1002/jssc.201601044</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of water on the retention on diol and amide columns in hydrophilic interaction liquid chromatography
Popis výsledku v původním jazyce
The amount of water adsorbed on polar columns plays important role in hydrophilic interaction liquid chromatography. It may strongly differ for the individual types of polar columns used in this separation mode. We measured adsorption isotherms of water on an amide and three diol-bonded stationary phases that differ in the chemistry of the bonded ligands and properties of the silica gel support. We studied the effects of the adsorbed water on the retention of aromatic carboxylic acids, flavonoids, benzoic acid derivatives, nucleic bases, and nucleosides in aqueous-acetonitrile mobile phases over the full composition range. The graphs of the retention factors versus the volume fraction of water in mobile phase show "U-profile" characteristic of a dual hydrophilic interaction-reversed phase retention mechanism. The minimum on the graph that marks the changing retention mechanism depends on the amount of adsorbed water. The linear solvation energy relationship model suggests that the retention in the hydrophilic interaction liquid chromatography mode is controlled mainly by proton-donor interactions in the stationary phase, depending on the column type. Finally, the accuracy of hydrophilic interaction liquid chromatography gradient prediction improves for columns that show a high water adsorption.
Název v anglickém jazyce
Effect of water on the retention on diol and amide columns in hydrophilic interaction liquid chromatography
Popis výsledku anglicky
The amount of water adsorbed on polar columns plays important role in hydrophilic interaction liquid chromatography. It may strongly differ for the individual types of polar columns used in this separation mode. We measured adsorption isotherms of water on an amide and three diol-bonded stationary phases that differ in the chemistry of the bonded ligands and properties of the silica gel support. We studied the effects of the adsorbed water on the retention of aromatic carboxylic acids, flavonoids, benzoic acid derivatives, nucleic bases, and nucleosides in aqueous-acetonitrile mobile phases over the full composition range. The graphs of the retention factors versus the volume fraction of water in mobile phase show "U-profile" characteristic of a dual hydrophilic interaction-reversed phase retention mechanism. The minimum on the graph that marks the changing retention mechanism depends on the amount of adsorbed water. The linear solvation energy relationship model suggests that the retention in the hydrophilic interaction liquid chromatography mode is controlled mainly by proton-donor interactions in the stationary phase, depending on the column type. Finally, the accuracy of hydrophilic interaction liquid chromatography gradient prediction improves for columns that show a high water adsorption.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Separation Science
ISSN
1615-9306
e-ISSN
—
Svazek periodika
40
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
15
Strana od-do
1434-1448
Kód UT WoS článku
000399782900001
EID výsledku v databázi Scopus
—