Recent advances in stationary phases and understanding of retention in hydrophilic interaction chromatography. A review

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39911193" target="_blank" >RIV/00216275:25310/17:39911193 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.aca.2017.01.060" target="_blank" >http://dx.doi.org/10.1016/j.aca.2017.01.060</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2017.01.060" target="_blank" >10.1016/j.aca.2017.01.060</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Recent advances in stationary phases and understanding of retention in hydrophilic interaction chromatography. A review

Popis výsledku v původním jazyce

Hydrophilic interaction chromatography (HILIC) is a rapidly developing liquid chromatography mode suitable for separation of strongly or moderately polar samples on polar columns in highly organic mixed mobile phases. In the past five years many new types of columns appeared based on silica gel, organic polymers and other types of supports. The present work reviews the new additions to their family. Further, the progress in the understanding of theoretical principles of the HILIC mode, especially of the role of the mobile phase, and of the adsorbed water are addressed. The amount of adsorbed water strongly depends on the type of the polar column and may be used to distinguish between &quot;conventional HILIC&quot; and &quot; aqueous normal phase chromatography (ANP)&quot;. Further, sample structural effects on the retention are treated in terms of the Linear Solvation Energy Relationship model adopted for partially ionized polar compounds. The impact of dual HILIC-reversed phase (RP) mechanism on a possible increase of the applications of some polar columns is discussed. Attention is paid also to the increasing role of the HILIC mode in various two-dimensional LC techniques and the related mobile phase compatibility problems.

Název v anglickém jazyce

Recent advances in stationary phases and understanding of retention in hydrophilic interaction chromatography. A review

Popis výsledku anglicky

Hydrophilic interaction chromatography (HILIC) is a rapidly developing liquid chromatography mode suitable for separation of strongly or moderately polar samples on polar columns in highly organic mixed mobile phases. In the past five years many new types of columns appeared based on silica gel, organic polymers and other types of supports. The present work reviews the new additions to their family. Further, the progress in the understanding of theoretical principles of the HILIC mode, especially of the role of the mobile phase, and of the adsorbed water are addressed. The amount of adsorbed water strongly depends on the type of the polar column and may be used to distinguish between &quot;conventional HILIC&quot; and &quot; aqueous normal phase chromatography (ANP)&quot;. Further, sample structural effects on the retention are treated in terms of the Linear Solvation Energy Relationship model adopted for partially ionized polar compounds. The impact of dual HILIC-reversed phase (RP) mechanism on a possible increase of the applications of some polar columns is discussed. Attention is paid also to the increasing role of the HILIC mode in various two-dimensional LC techniques and the related mobile phase compatibility problems.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Dvourozměrné separace v kapalných médiích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

—

Svazek periodika

967

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

21

Strana od-do

12-32

Kód UT WoS článku

000398436000002

EID výsledku v databázi Scopus

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