A high-performance liquid chromatography method with pre-column derivatization for the simultaneous determination of reduced glutathione, carnosine and taurine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F14%3A10282243" target="_blank" >RIV/00216208:11160/14:10282243 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.rsc.org/en/content/articlehtml/2014/ay/c3ay42200c" target="_blank" >http://pubs.rsc.org/en/content/articlehtml/2014/ay/c3ay42200c</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c3ay42200c" target="_blank" >10.1039/c3ay42200c</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A high-performance liquid chromatography method with pre-column derivatization for the simultaneous determination of reduced glutathione, carnosine and taurine

Popis výsledku v původním jazyce

A new gradient reversed-phase HPLC method with pre-column derivatization was developed and validated for simultaneous determination of reduced glutathione, carnosine and taurine in new pharmaceutical eye drops. Chromatographic separation was achieved with a fused core analytical column Supelco Ascentis Express C18 (100 mm x 4.6 mm, 2.7 mu m). A 30 mM triethylamine in acetonitrile with 30 mM triethylamine aqueous solution (pH 2.5, adjusted with glacial acetic acid) was used in a gradient elution mode ata flow rate of 0.8 mL min(-1). The column temperature was maintained at 30 degrees C, the injection volume of the derivatized sample was 5 mu L and the detection wavelength was 375 nm. The tested validation parameters included system suitability, accuracy, precision, linearity, LOD, LOQ and sample solution stability. The standard calibration curves showed good linearity with correlation coefficients r > 0.997 for all compounds. The mean percentage recoveries obtained for reduced glutathi

Název v anglickém jazyce

A high-performance liquid chromatography method with pre-column derivatization for the simultaneous determination of reduced glutathione, carnosine and taurine

Popis výsledku anglicky

A new gradient reversed-phase HPLC method with pre-column derivatization was developed and validated for simultaneous determination of reduced glutathione, carnosine and taurine in new pharmaceutical eye drops. Chromatographic separation was achieved with a fused core analytical column Supelco Ascentis Express C18 (100 mm x 4.6 mm, 2.7 mu m). A 30 mM triethylamine in acetonitrile with 30 mM triethylamine aqueous solution (pH 2.5, adjusted with glacial acetic acid) was used in a gradient elution mode ata flow rate of 0.8 mL min(-1). The column temperature was maintained at 30 degrees C, the injection volume of the derivatized sample was 5 mu L and the detection wavelength was 375 nm. The tested validation parameters included system suitability, accuracy, precision, linearity, LOD, LOQ and sample solution stability. The standard calibration curves showed good linearity with correlation coefficients r > 0.997 for all compounds. The mean percentage recoveries obtained for reduced glutathi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.20.0235" target="_blank" >EE2.3.20.0235: Vybudování výzkumného týmu experimentální a aplikované biofarmacie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods

ISSN

1759-9660

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

1475-1481

Kód UT WoS článku

000332129500030

EID výsledku v databázi Scopus

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