Micro-extraction by packed sorbent coupled on-line to a column-switching chromatography system - A case study on the determination of three beta-blockers in human urine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F19%3A10400802" target="_blank" >RIV/00216208:11160/19:10400802 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=dHh-mEw5tS" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=dHh-mEw5tS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2019.02.069" target="_blank" >10.1016/j.microc.2019.02.069</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Micro-extraction by packed sorbent coupled on-line to a column-switching chromatography system - A case study on the determination of three beta-blockers in human urine

Popis výsledku v původním jazyce

A new approach for the automation of Micro-Extraction by Packed Sorbent (MEPS) as a solid phase extraction technique coupled directly to a column-switching chromatography system was presented. A new on-line hyphenation of the modern micro-extraction procedure with liquid chromatography was developed and used for human urine sample clean-up and pre-concentration of three beta-blockers - metoprolol, labetalol and propranolol before separation and determination. A commercial MEPS C-18 cartridge was coupled using Teflon holders into an HPLC system through a high-pressure six-port switching valve. Chromatographic separation was performed on a Triart YMC C-18 (50 mm x 4.6 mm) analytical column with 5 mu m multi-layered particles. The multilayered particle column showed extremely low flow-resistance (&lt; 4.1 MPa) at a flow rate of 1.0 mL min(-1), and thus direct connection with a MEPS cartridge was possible. A mixture of acetonitrile and an aqueous solution of 0.5% triethylamine with acetic acid, with pH adjusted to 4.5 in the linear gradient elution mode was used. This mobile phase eluted beta-blockers from the MEPS mini-column directly onto the analytical column where the separation was carried out. Metoprolol, labetalol and propranolol were detected by a fluorescence detector at the lambda(ex) = 220 nm and lambda(em) = 315 nm wavelengths. The developed on-line MEPS-HPLC coupling showed satisfactory validation results with recovery ranging from 94.0 to 104.7% and precision in the range of 0.6-9.5% for all beta-blockers.

Název v anglickém jazyce

Micro-extraction by packed sorbent coupled on-line to a column-switching chromatography system - A case study on the determination of three beta-blockers in human urine

Popis výsledku anglicky

A new approach for the automation of Micro-Extraction by Packed Sorbent (MEPS) as a solid phase extraction technique coupled directly to a column-switching chromatography system was presented. A new on-line hyphenation of the modern micro-extraction procedure with liquid chromatography was developed and used for human urine sample clean-up and pre-concentration of three beta-blockers - metoprolol, labetalol and propranolol before separation and determination. A commercial MEPS C-18 cartridge was coupled using Teflon holders into an HPLC system through a high-pressure six-port switching valve. Chromatographic separation was performed on a Triart YMC C-18 (50 mm x 4.6 mm) analytical column with 5 mu m multi-layered particles. The multilayered particle column showed extremely low flow-resistance (&lt; 4.1 MPa) at a flow rate of 1.0 mL min(-1), and thus direct connection with a MEPS cartridge was possible. A mixture of acetonitrile and an aqueous solution of 0.5% triethylamine with acetic acid, with pH adjusted to 4.5 in the linear gradient elution mode was used. This mobile phase eluted beta-blockers from the MEPS mini-column directly onto the analytical column where the separation was carried out. Metoprolol, labetalol and propranolol were detected by a fluorescence detector at the lambda(ex) = 220 nm and lambda(em) = 315 nm wavelengths. The developed on-line MEPS-HPLC coupling showed satisfactory validation results with recovery ranging from 94.0 to 104.7% and precision in the range of 0.6-9.5% for all beta-blockers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA17-08738S" target="_blank" >GA17-08738S: Nanovlákenné polymery jako perspektivní sorbenty pro on-line extrakci v chromatografických systémech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

—

Svazek periodika

147

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

60-66

Kód UT WoS článku

000468714400008

EID výsledku v databázi Scopus

2-s2.0-85062490103