Rapid Determination of alpha-Hederin and Hederacoside C in Extracts of Hedera helix Leaves Available in the Czech Republic and Poland

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F15%3A10312549" target="_blank" >RIV/00216208:11160/15:10312549 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Rapid Determination of alpha-Hederin and Hederacoside C in Extracts of Hedera helix Leaves Available in the Czech Republic and Poland

Popis výsledku v původním jazyce

Leaf extracts of Hedera helix L. are widely used in the treatment of upper respiratory diseases. The saponins alpha-hederin and hederacoside C are considered to be the main compounds responsible for the biological activity. alpha-Hederin and hederacosideC were determined in H. helix leaf extracts using a fast, simple and validated HPLC method. An XTerra MS C18 column and mobile phase composed of 10 mM ammonium acetate at pH 8.5 (adjusted with triethylamine) and acetonitrile were used for the chromatography at 1.2 ml min(-1). The column was kept at 30 degrees C. Detection was performed at 220 nm. An approach utilizing a basic pH of the aqueous part of the mobile phase enabled analysis in 5 minutes in isocratic mode. The method was validated and used for the quality control of H. helix leaf ethanolic extracts.

Název v anglickém jazyce

Rapid Determination of alpha-Hederin and Hederacoside C in Extracts of Hedera helix Leaves Available in the Czech Republic and Poland

Popis výsledku anglicky

Leaf extracts of Hedera helix L. are widely used in the treatment of upper respiratory diseases. The saponins alpha-hederin and hederacoside C are considered to be the main compounds responsible for the biological activity. alpha-Hederin and hederacosideC were determined in H. helix leaf extracts using a fast, simple and validated HPLC method. An XTerra MS C18 column and mobile phase composed of 10 mM ammonium acetate at pH 8.5 (adjusted with triethylamine) and acetonitrile were used for the chromatography at 1.2 ml min(-1). The column was kept at 30 degrees C. Detection was performed at 220 nm. An approach utilizing a basic pH of the aqueous part of the mobile phase enabled analysis in 5 minutes in isocratic mode. The method was validated and used for the quality control of H. helix leaf ethanolic extracts.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA15-10781S" target="_blank" >GA15-10781S: On-line spojení automatizovaných extrakčních procesů s kapalinovou chromatografií pro kompletní analýzu vzorku</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Natural Product Communications

ISSN

1934-578X

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

1529-1531

Kód UT WoS článku

000361868900010

EID výsledku v databázi Scopus

2-s2.0-84954614057