Stimulation of PPAR alpha normalizes the skin lipid ratio and improves the skin barrier of normal and filaggrin deficient reconstructed skin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F15%3A10322892" target="_blank" >RIV/00216208:11160/15:10322892 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0923181115300529" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0923181115300529</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jdermsci.2015.09.012" target="_blank" >10.1016/j.jdermsci.2015.09.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stimulation of PPAR alpha normalizes the skin lipid ratio and improves the skin barrier of normal and filaggrin deficient reconstructed skin

Popis výsledku v původním jazyce

Background: Therapeutic options for atopic dermatitis mostly address the symptoms but causal therapies are still missing. Peroxisome proliferator activated receptor (PPAR) agonists exert beneficial effects in patients suffering this disease, whereas thestimulation of PPAR alpha and gamma seemed most promising. Objectives: To elucidate the effects of the PPARa specific agonist WY14643, the PPAR gamma agonist ciglitazone, and the dual PPAR alpha + gamma agonist docosahexaenoic acid (DHA) on the homeostasis and barrier function of filaggrin deficient skin. Methods: The effects of the PPAR agonists on skin differentiation were evaluated via qPCR, Western blot, histological or immunofluorescence staining. Skin lipid organization was determined by ATR-FTIRand lipid composition was analyzed by HPTLC. Ultimately, the skin barrier function was assessed by skin absorption studies using the radioactively labeled compound testosterone. Results: Significant upregulation of filaggrin after DHA and

Název v anglickém jazyce

Stimulation of PPAR alpha normalizes the skin lipid ratio and improves the skin barrier of normal and filaggrin deficient reconstructed skin

Popis výsledku anglicky

Background: Therapeutic options for atopic dermatitis mostly address the symptoms but causal therapies are still missing. Peroxisome proliferator activated receptor (PPAR) agonists exert beneficial effects in patients suffering this disease, whereas thestimulation of PPAR alpha and gamma seemed most promising. Objectives: To elucidate the effects of the PPARa specific agonist WY14643, the PPAR gamma agonist ciglitazone, and the dual PPAR alpha + gamma agonist docosahexaenoic acid (DHA) on the homeostasis and barrier function of filaggrin deficient skin. Methods: The effects of the PPAR agonists on skin differentiation were evaluated via qPCR, Western blot, histological or immunofluorescence staining. Skin lipid organization was determined by ATR-FTIRand lipid composition was analyzed by HPTLC. Ultimately, the skin barrier function was assessed by skin absorption studies using the radioactively labeled compound testosterone. Results: Significant upregulation of filaggrin after DHA and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA13-23891S" target="_blank" >GA13-23891S: Modely lipidových membrán - nový nástroj pro studium patofyziologie kožních onemocnění na molekulární úrovni</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Dermatological Science

ISSN

0923-1811

e-ISSN

—

Svazek periodika

80

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

9

Strana od-do

102-110

Kód UT WoS článku

000366882400004

EID výsledku v databázi Scopus

2-s2.0-84949220990