Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity on Microstructure and Permeability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F16%3A10335281" target="_blank" >RIV/00216208:11160/16:10335281 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.acs.org/doi/full/10.1021/acs.langmuir.6b03082" target="_blank" >http://pubs.acs.org/doi/full/10.1021/acs.langmuir.6b03082</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.langmuir.6b03082" target="_blank" >10.1021/acs.langmuir.6b03082</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity on Microstructure and Permeability

Popis výsledku v původním jazyce

Omega-O-acylceramides (acylCer), a subclass of sphingolipids with an ultralong N-acyl chain (from 20 to 38 carbons, most usually 30 and 32 carbons), are crucial components of the skin permeability barrier. AcylCer are involved in the formation of the long periodicity lamellar phase (LPP, 12-13 nm), which is essential for preventing water loss from the body. Lower levels of acylCer and LPP accompany skin diseases, such as atopic dermatitis, lamellar ichthyosis, and psoriasis. We studied how the concentration and structure of acylCer influence the organization and permeability barrier properties of model lipid membranes. For simple model membranes composed of the sphingosine-containing acylCer (EOS), N-lignoceroyl sphingosine, lignoceric acid, cholesterol (Chol), and cholesteryl sulfate (CholS), the LPP formed at 10% Cer EOS (of the total Cer) and the short periodicity phase disappeared at 30% Cer EOS. Surprisingly, membranes with the LPP had higher permeabilities than the control membrane without acylCer. In the complex models consisting of acylCer (EOS, phytosphingosine EOP, dihydrosphingosine EOdS, or their mixture; at 10% of the total Cer), a six-component Cer mixture, a free fatty acid mixture, cholesterol (Chol), and cholesteryl sulfate (CholS), acylCer decreased the membrane permeability to model permeants (with the strongest effects for acylCer EOP and EOdS) when compared with the permeability of the control membrane without acylCer. However, in the complex model, only a mixture of acylCer EOS, EOdS, and EOP and not the individual acylCer formed both the LPP and orthorhombic chain packing at the 10% level. Thus, the relationships between acylCer, LPP formation, and permeability barrier function are not trivial. Lipid heterogeneity is essential-only the most complex model with nine Cer subclasses mimicked both the organization and permeability of stratum corneum lipid membranes.

Název v anglickém jazyce

Omega-O-Acylceramides in Skin Lipid Membranes: Effects of Concentration, Sphingoid Base, and Model Complexity on Microstructure and Permeability

Popis výsledku anglicky

Omega-O-acylceramides (acylCer), a subclass of sphingolipids with an ultralong N-acyl chain (from 20 to 38 carbons, most usually 30 and 32 carbons), are crucial components of the skin permeability barrier. AcylCer are involved in the formation of the long periodicity lamellar phase (LPP, 12-13 nm), which is essential for preventing water loss from the body. Lower levels of acylCer and LPP accompany skin diseases, such as atopic dermatitis, lamellar ichthyosis, and psoriasis. We studied how the concentration and structure of acylCer influence the organization and permeability barrier properties of model lipid membranes. For simple model membranes composed of the sphingosine-containing acylCer (EOS), N-lignoceroyl sphingosine, lignoceric acid, cholesterol (Chol), and cholesteryl sulfate (CholS), the LPP formed at 10% Cer EOS (of the total Cer) and the short periodicity phase disappeared at 30% Cer EOS. Surprisingly, membranes with the LPP had higher permeabilities than the control membrane without acylCer. In the complex models consisting of acylCer (EOS, phytosphingosine EOP, dihydrosphingosine EOdS, or their mixture; at 10% of the total Cer), a six-component Cer mixture, a free fatty acid mixture, cholesterol (Chol), and cholesteryl sulfate (CholS), acylCer decreased the membrane permeability to model permeants (with the strongest effects for acylCer EOP and EOdS) when compared with the permeability of the control membrane without acylCer. However, in the complex model, only a mixture of acylCer EOS, EOdS, and EOP and not the individual acylCer formed both the LPP and orthorhombic chain packing at the 10% level. Thus, the relationships between acylCer, LPP formation, and permeability barrier function are not trivial. Lipid heterogeneity is essential-only the most complex model with nine Cer subclasses mimicked both the organization and permeability of stratum corneum lipid membranes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GC16-25687J" target="_blank" >GC16-25687J: Vztahy mezi zánětlivými procesy a bariérovými lipidy u onemocnění kůže</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Langmuir

ISSN

0743-7463

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

48

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

12894-12904

Kód UT WoS článku

000389557400038

EID výsledku v databázi Scopus

2-s2.0-85002486989