Permeability and microstructure of model stratum corneum lipid membranes containing ceramides with long (C16) and very long (C24) acyl chains

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10364892" target="_blank" >RIV/00216208:11160/17:10364892 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22310/17:43914226 RIV/60461373:22810/17:43914226

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S030146221630463X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S030146221630463X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bpc.2017.03.004" target="_blank" >10.1016/j.bpc.2017.03.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Permeability and microstructure of model stratum corneum lipid membranes containing ceramides with long (C16) and very long (C24) acyl chains

Popis výsledku v původním jazyce

The Stratum corneum (SC) prevents water loss from the body and absorption of chemicals. SC intercellular spaces contain ceramides (Cer), free fatty acids (FFA), cholesterol (Chol) and cholesteryl sulfate (CholS). Cer with &quot;very long&quot; acyl chains (for example, N-lignoceroyl-sphingosine, CerNS24) are important for skin barrier function, whereas increased levels of &quot;long&quot; acyl Cer (for example, N-palmitoyl-sphingosine, CerNS16) occur in patients suffering from atopic eczema or psoriasis. We studied the impact of the replacement of CerNS24 by CerNS16 on the barrier properties and microstructure of model SC lipid membranes composed of Cer/FFA/Chol/CholS. Membranes containing the long CerNS16 were significantly more permeable to water (by 38-53%), theophylline (by 50-55%) and indomethacin (by 83-120%) than those containing the very long CerNS24 (either with lignoceric acid or a mixture of long to very long chain FFA). Langmuir monolayers with CerNS24 were more condensed than with CerNS16 and atomic force microscopy showed differences in domain formation. X-ray powder diffraction revealed that CerNS24-based membranes formed one lamellar phase and separated Chol, whereas the CerNS16-based membranes formed up to three phases and Chol. These results suggest that replacement of CerNS24 by CerNS16 has a direct negative impact on membrane structure and permeability.

Název v anglickém jazyce

Permeability and microstructure of model stratum corneum lipid membranes containing ceramides with long (C16) and very long (C24) acyl chains

Popis výsledku anglicky

The Stratum corneum (SC) prevents water loss from the body and absorption of chemicals. SC intercellular spaces contain ceramides (Cer), free fatty acids (FFA), cholesterol (Chol) and cholesteryl sulfate (CholS). Cer with &quot;very long&quot; acyl chains (for example, N-lignoceroyl-sphingosine, CerNS24) are important for skin barrier function, whereas increased levels of &quot;long&quot; acyl Cer (for example, N-palmitoyl-sphingosine, CerNS16) occur in patients suffering from atopic eczema or psoriasis. We studied the impact of the replacement of CerNS24 by CerNS16 on the barrier properties and microstructure of model SC lipid membranes composed of Cer/FFA/Chol/CholS. Membranes containing the long CerNS16 were significantly more permeable to water (by 38-53%), theophylline (by 50-55%) and indomethacin (by 83-120%) than those containing the very long CerNS24 (either with lignoceric acid or a mixture of long to very long chain FFA). Langmuir monolayers with CerNS24 were more condensed than with CerNS16 and atomic force microscopy showed differences in domain formation. X-ray powder diffraction revealed that CerNS24-based membranes formed one lamellar phase and separated Chol, whereas the CerNS16-based membranes formed up to three phases and Chol. These results suggest that replacement of CerNS24 by CerNS16 has a direct negative impact on membrane structure and permeability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biophysical Chemistry

ISSN

0301-4622

e-ISSN

—

Svazek periodika

224

Číslo periodika v rámci svazku

May

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

20-31

Kód UT WoS článku

000401597100002

EID výsledku v databázi Scopus

2-s2.0-85016137333