A UHPLC method for the rapid separation and quantification of anthocyanins in acai berry and dry blueberry extracts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10362176" target="_blank" >RIV/00216208:11160/17:10362176 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0731708517309421" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0731708517309421</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpba.2017.05.045" target="_blank" >10.1016/j.jpba.2017.05.045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A UHPLC method for the rapid separation and quantification of anthocyanins in acai berry and dry blueberry extracts

Popis výsledku v původním jazyce

The presented work describes the development and validation of a rapid UHPLC-UV method using a core-shell particle column with a pentafluorophenyl stationary phase for the separation and quantitative analysis of the six anthocyanins in acai berry and dry blueberry extracts. The anthocyanins (cyanidin-3-glucoside, cyanidin-3-rutenoside, delphinidin-3-galactoside, delphinidin-3-glucoside, delphinidin-3-rutenoside, and peonidin-3-glucoside) were separated and analyzed in 5 min. The chromatographic separation was performed on a Kinetex PFP (150 x 2.1 mm) core-shell column with a particle size of 1.7 mu m at a temperature of 50 degrees C. Acetonitrile was used as mobile phase B and 5% formic acid, filtrated through a 0.22 mu m filter, as mobile phase A. They were delivered at a flow rate of 0.55 mL min(-1) according to the elution gradient program. The detection wavelength was set at 520 nm. A solid-liquid extraction with a solution of methanol and a 5% water solution of formic acid (25+75 v/v) using an ultrasonic bath was chosen for the preparation of the available commercial samples of food supplements with a content of acai berry extract and blueberry extract. Under optimal chromatographic conditions, the method was validated. Recoveries for all analyzed anthocyanins were 97.8-102.6% and the relative standard deviation ranged from 0.4% to 3.0% for within-day and from 0.6% to 3.1% for between-day repeatability. The limits of detection were in the range of 0.11-0.14 mu g mL(-1).

Název v anglickém jazyce

A UHPLC method for the rapid separation and quantification of anthocyanins in acai berry and dry blueberry extracts

Popis výsledku anglicky

The presented work describes the development and validation of a rapid UHPLC-UV method using a core-shell particle column with a pentafluorophenyl stationary phase for the separation and quantitative analysis of the six anthocyanins in acai berry and dry blueberry extracts. The anthocyanins (cyanidin-3-glucoside, cyanidin-3-rutenoside, delphinidin-3-galactoside, delphinidin-3-glucoside, delphinidin-3-rutenoside, and peonidin-3-glucoside) were separated and analyzed in 5 min. The chromatographic separation was performed on a Kinetex PFP (150 x 2.1 mm) core-shell column with a particle size of 1.7 mu m at a temperature of 50 degrees C. Acetonitrile was used as mobile phase B and 5% formic acid, filtrated through a 0.22 mu m filter, as mobile phase A. They were delivered at a flow rate of 0.55 mL min(-1) according to the elution gradient program. The detection wavelength was set at 520 nm. A solid-liquid extraction with a solution of methanol and a 5% water solution of formic acid (25+75 v/v) using an ultrasonic bath was chosen for the preparation of the available commercial samples of food supplements with a content of acai berry extract and blueberry extract. Under optimal chromatographic conditions, the method was validated. Recoveries for all analyzed anthocyanins were 97.8-102.6% and the relative standard deviation ranged from 0.4% to 3.0% for within-day and from 0.6% to 3.1% for between-day repeatability. The limits of detection were in the range of 0.11-0.14 mu g mL(-1).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Pharmaceutical and Biomedical Analysis

ISSN

0731-7085

e-ISSN

—

Svazek periodika

143

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

204-213

Kód UT WoS článku

000405537300027

EID výsledku v databázi Scopus

2-s2.0-85020667534