Comparison of nano and conventional liquid chromatographic methods for the separation of ( )-catechin-ethyl-malvidin-3-glucoside diastereoisomers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33161047" target="_blank" >RIV/61989592:15310/16:33161047 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0021967315013941" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967315013941</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2015.09.074" target="_blank" >10.1016/j.chroma.2015.09.074</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of nano and conventional liquid chromatographic methods for the separation of ( )-catechin-ethyl-malvidin-3-glucoside diastereoisomers

Popis výsledku v původním jazyce

Nano-liquid chromatography and conventional HPLC were used for the separation of diastereomers of (+)-catechin-ethyl-malvidin-3-glucoside. Those bridged anthocyanin dyes were obtained by reaction of (+)-catechin with malvidin-3-glucoside in the presence of acetaldehyde. Both diastereomers were isolated with semipreparative chromatography and their structures were confirmed by nuclear magnetic resonance and mass spectrometry. In-laboratory prepared capillary columns packed with fully porous particles Chromosphere C18, dp = 3 mu m, core-shell particles Kinetex C18, dp = 2.6 mu m (100 mu m i.d.) and monolithic column Chromolith CapRod (100 mu m i.d.) were used for the separation of (+)-catechin, malvidin-3-glucoside and both diastereomers. Chromosphere C18 stationary phase provided the best chromatographic performance. Mobile phase containing water:acetonitrile (80:20) acidified with trifluoroacetic acid (0.1%, v/v/v) was used in an isocratic elution mode with a flow rate of 360 nL min(-1). Separation of studied compounds was achieved in less than 7 min under optimized conditions. The nano-liquid chromatographic method and a conventional HPLC one using the same fully porous particles (Chromosphere C18, 3 mu m, 100 mm x 4.6 mm) were compared providing higher separation efficiency with the first analytical method and similar selectivity. A better peak symmetry and higher resolution of the studied diastereomers was achieved by conventional chromatography. Nevertheless, nano-liquid chromatography appeared to be useful for the separation of complex anthocyanin dyes and can be utilized for their analysis in plant and food micro-samples. The developed method was used for analysis of red wine grape pomace

Název v anglickém jazyce

Comparison of nano and conventional liquid chromatographic methods for the separation of ( )-catechin-ethyl-malvidin-3-glucoside diastereoisomers

Popis výsledku anglicky

Nano-liquid chromatography and conventional HPLC were used for the separation of diastereomers of (+)-catechin-ethyl-malvidin-3-glucoside. Those bridged anthocyanin dyes were obtained by reaction of (+)-catechin with malvidin-3-glucoside in the presence of acetaldehyde. Both diastereomers were isolated with semipreparative chromatography and their structures were confirmed by nuclear magnetic resonance and mass spectrometry. In-laboratory prepared capillary columns packed with fully porous particles Chromosphere C18, dp = 3 mu m, core-shell particles Kinetex C18, dp = 2.6 mu m (100 mu m i.d.) and monolithic column Chromolith CapRod (100 mu m i.d.) were used for the separation of (+)-catechin, malvidin-3-glucoside and both diastereomers. Chromosphere C18 stationary phase provided the best chromatographic performance. Mobile phase containing water:acetonitrile (80:20) acidified with trifluoroacetic acid (0.1%, v/v/v) was used in an isocratic elution mode with a flow rate of 360 nL min(-1). Separation of studied compounds was achieved in less than 7 min under optimized conditions. The nano-liquid chromatographic method and a conventional HPLC one using the same fully porous particles (Chromosphere C18, 3 mu m, 100 mm x 4.6 mm) were compared providing higher separation efficiency with the first analytical method and similar selectivity. A better peak symmetry and higher resolution of the studied diastereomers was achieved by conventional chromatography. Nevertheless, nano-liquid chromatography appeared to be useful for the separation of complex anthocyanin dyes and can be utilized for their analysis in plant and food micro-samples. The developed method was used for analysis of red wine grape pomace

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A: Symposium Volumes

ISSN

0021-9673

e-ISSN

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Svazek periodika

1428

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

126-133

Kód UT WoS článku

000368564000010

EID výsledku v databázi Scopus

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