Multilayered particle-packed column: Evaluation and comparison with monolithic and core-shell particle columns for the determination of red azo dyes in Sequential Injection Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10364765" target="_blank" >RIV/00216208:11160/17:10364765 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201601224" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201601224</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201601224" target="_blank" >10.1002/jssc.201601224</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multilayered particle-packed column: Evaluation and comparison with monolithic and core-shell particle columns for the determination of red azo dyes in Sequential Injection Chromatography

Popis výsledku v původním jazyce

A recently presented new type of &quot;multilayered&quot; organic-inorganic hybrid silica particle packed column YMC-Triart C-18 (50 mm x 4.6 mm, 5 mu m) was used for the development of a sequential injection chromatography method for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orange G, and para red) in selected food seasonings. The use of a novel sorbent brings attractive features, reduced backpressure, and broader chemical stability together with high separation performance, which are discussed and compared with that of three types of columns typically used in medium-pressure flow chromatography techniques (classic monolithic, narrow monolithic, and core-shell particle columns). The separation was performed in gradient elution mode created by the zone mixing of two mobile phases (acetonitrile/water 90:10, 1.5 mL + acetonitrile/water 100:0, 2.3 mL) at a flow rate of 0.60 mL/min and time of analysis &lt; 9.5 min. The spectrophotometric detection wavelengths were set to 400, 480, and 500 nm. The high performance of the developed method with multilayered particle column was well documented and the results indicate a broad capability of sequential injection chromatography.

Název v anglickém jazyce

Multilayered particle-packed column: Evaluation and comparison with monolithic and core-shell particle columns for the determination of red azo dyes in Sequential Injection Chromatography

Popis výsledku anglicky

A recently presented new type of &quot;multilayered&quot; organic-inorganic hybrid silica particle packed column YMC-Triart C-18 (50 mm x 4.6 mm, 5 mu m) was used for the development of a sequential injection chromatography method for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orange G, and para red) in selected food seasonings. The use of a novel sorbent brings attractive features, reduced backpressure, and broader chemical stability together with high separation performance, which are discussed and compared with that of three types of columns typically used in medium-pressure flow chromatography techniques (classic monolithic, narrow monolithic, and core-shell particle columns). The separation was performed in gradient elution mode created by the zone mixing of two mobile phases (acetonitrile/water 90:10, 1.5 mL + acetonitrile/water 100:0, 2.3 mL) at a flow rate of 0.60 mL/min and time of analysis &lt; 9.5 min. The spectrophotometric detection wavelengths were set to 400, 480, and 500 nm. The high performance of the developed method with multilayered particle column was well documented and the results indicate a broad capability of sequential injection chromatography.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/GA15-10781S" target="_blank" >GA15-10781S: On-line spojení automatizovaných extrakčních procesů s kapalinovou chromatografií pro kompletní analýzu vzorku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

1225-1233

Kód UT WoS článku

000397830200001

EID výsledku v databázi Scopus

2-s2.0-85013884505