On-line coupling of molecularly imprinted solid phase extraction with liquid chromatography for the fast determination of coumarins from complex samples
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10365191" target="_blank" >RIV/00216208:11160/17:10365191 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S073170851730941X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S073170851730941X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2017.06.033" target="_blank" >10.1016/j.jpba.2017.06.033</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
On-line coupling of molecularly imprinted solid phase extraction with liquid chromatography for the fast determination of coumarins from complex samples
Popis výsledku v původním jazyce
In this work, an on-line SPE-HPLC method with spectrophotometric detection was developed for the determination of coumarins in complex samples. For the on-line cleanup of samples, a molecularly imprinted polymer was packed into the column cartridge and coupled directly with HPLC (MISPE-HPLC) using a column switching system. The separation of coumarins was performed on a C18 core-shell column (100 x 4.6 mm, 5 mu m) with a mobile phase consisting of 0.3% acetic acid/acetonitrile with gradient elution at a flow-rate of 1 mLmin(-1). The total time of the whole analytical run, including the extraction step, was 13.25 min. The on-line MISPE-HPLC method was optimized and validated. The results showed good linearity (0.10-100 mu g mL(-1)) with correlation coefficients higher than 0.99. The LOD values were from 0.03 to 0.15 mu gmL(-1). The proposed method was successfully applied for analysis of real samples (Cassia cinnamon, chamomile tea, and Tokaj specialty wines) and obtained recoveries varied from 78.7% to 112.2% with an RED less than 9%.
Název v anglickém jazyce
On-line coupling of molecularly imprinted solid phase extraction with liquid chromatography for the fast determination of coumarins from complex samples
Popis výsledku anglicky
In this work, an on-line SPE-HPLC method with spectrophotometric detection was developed for the determination of coumarins in complex samples. For the on-line cleanup of samples, a molecularly imprinted polymer was packed into the column cartridge and coupled directly with HPLC (MISPE-HPLC) using a column switching system. The separation of coumarins was performed on a C18 core-shell column (100 x 4.6 mm, 5 mu m) with a mobile phase consisting of 0.3% acetic acid/acetonitrile with gradient elution at a flow-rate of 1 mLmin(-1). The total time of the whole analytical run, including the extraction step, was 13.25 min. The on-line MISPE-HPLC method was optimized and validated. The results showed good linearity (0.10-100 mu g mL(-1)) with correlation coefficients higher than 0.99. The LOD values were from 0.03 to 0.15 mu gmL(-1). The proposed method was successfully applied for analysis of real samples (Cassia cinnamon, chamomile tea, and Tokaj specialty wines) and obtained recoveries varied from 78.7% to 112.2% with an RED less than 9%.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30104 - Pharmacology and pharmacy
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085
e-ISSN
—
Svazek periodika
145
Číslo periodika v rámci svazku
October
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
7
Strana od-do
144-150
Kód UT WoS článku
000410872200019
EID výsledku v databázi Scopus
2-s2.0-85021375380