"Single-pot" approach towards the preparation of alkyl and polyfluoroalkyl organo-silica monolithic capillaries for reversed-phase liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10382540" target="_blank" >RIV/00216208:11160/18:10382540 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201800688" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201800688</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201800688" target="_blank" >10.1002/jssc.201800688</a>

Jazyk výsledku

angličtina

Název v původním jazyce

"Single-pot" approach towards the preparation of alkyl and polyfluoroalkyl organo-silica monolithic capillaries for reversed-phase liquid chromatography

Popis výsledku v původním jazyce

Organo-silica monolithic capillary columns were prepared using thermally initiated polymerization of mixtures containing 3-(methacryloyloxy)propyltrimethoxysilane as the silicon-containing monomer, an aqueous acid catalyst, ethylene dimethacrylate as the cross-linker, a functional monomer selected from the group comprising octadecyl methacrylate, trifluoroethyl methacrylate, pentafluoropropyl methacrylate, and heptadecafluorodecyl methacrylate, toluene as the porogen, and 2,2&apos;-azobisisobutyronitrile as the initiator. The permeability of the monoliths was controlled by varying the composition of the polymerization mixture. Chromatographic properties of resulting monolithic 100 mu m i.d. capillaries were evaluated utilizing four sets of probe compounds under isocratic elution conditions in the reversed-phase mode. Baseline separation of alkylbenzenes and the best steric selectivity were achieved using the octadecyl methacrylate monolithic column verifying that its surface was the most hydrophobic. In contrast, separation of a mixture containing polar compounds was observed using columns prepared in the absence of the functional monomer due to the presence of residual surface silanols at the pore surface. Polyfluoroalkyl stationary phases, especially those with a high number of fluorine atoms, exhibited the highest selectivity towards fluorine-containing analytes.

Název v anglickém jazyce

"Single-pot" approach towards the preparation of alkyl and polyfluoroalkyl organo-silica monolithic capillaries for reversed-phase liquid chromatography

Popis výsledku anglicky

Organo-silica monolithic capillary columns were prepared using thermally initiated polymerization of mixtures containing 3-(methacryloyloxy)propyltrimethoxysilane as the silicon-containing monomer, an aqueous acid catalyst, ethylene dimethacrylate as the cross-linker, a functional monomer selected from the group comprising octadecyl methacrylate, trifluoroethyl methacrylate, pentafluoropropyl methacrylate, and heptadecafluorodecyl methacrylate, toluene as the porogen, and 2,2&apos;-azobisisobutyronitrile as the initiator. The permeability of the monoliths was controlled by varying the composition of the polymerization mixture. Chromatographic properties of resulting monolithic 100 mu m i.d. capillaries were evaluated utilizing four sets of probe compounds under isocratic elution conditions in the reversed-phase mode. Baseline separation of alkylbenzenes and the best steric selectivity were achieved using the octadecyl methacrylate monolithic column verifying that its surface was the most hydrophobic. In contrast, separation of a mixture containing polar compounds was observed using columns prepared in the absence of the functional monomer due to the presence of residual surface silanols at the pore surface. Polyfluoroalkyl stationary phases, especially those with a high number of fluorine atoms, exhibited the highest selectivity towards fluorine-containing analytes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

3669-3676

Kód UT WoS článku

000446667500002

EID výsledku v databázi Scopus

2-s2.0-85052380927