DNA-DOPE-gemini surfactants complexes at low surface charge density: from structure to transfection efficiency

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10393269" target="_blank" >RIV/00216208:11160/18:10393269 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.elis.sk/download_file.php?product_id=5546" target="_blank" >http://www.elis.sk/download_file.php?product_id=5546</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/gpb_2017042" target="_blank" >10.4149/gpb_2017042</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA-DOPE-gemini surfactants complexes at low surface charge density: from structure to transfection efficiency

Popis výsledku v původním jazyce

DNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-alpha,omega-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45-60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy. The in vitro transfection efficiency of CnGS12/DOPE liposomes on mammalian HEK 293 cell line was spacer length-dependent. C12GS12/DOPE/DNA complexes exhibited the best transfection efficiency (~ 18% GFP-expressing cells relative to all viable cells) accompanied by ~ 89% cell viability.

Název v anglickém jazyce

DNA-DOPE-gemini surfactants complexes at low surface charge density: from structure to transfection efficiency

Popis výsledku anglicky

DNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-alpha,omega-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45-60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy. The in vitro transfection efficiency of CnGS12/DOPE liposomes on mammalian HEK 293 cell line was spacer length-dependent. C12GS12/DOPE/DNA complexes exhibited the best transfection efficiency (~ 18% GFP-expressing cells relative to all viable cells) accompanied by ~ 89% cell viability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

General Physiology and Biophysics

ISSN

0231-5882

e-ISSN

—

Svazek periodika

37

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

13

Strana od-do

57-69

Kód UT WoS článku

000425206200006

EID výsledku v databázi Scopus

2-s2.0-85044594233