Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F15%3A43872977" target="_blank" >RIV/70883521:28610/15:43872977 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs10529-014-1727-7" target="_blank" >http://link.springer.com/article/10.1007%2Fs10529-014-1727-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10529-014-1727-7" target="_blank" >10.1007/s10529-014-1727-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Popis výsledku v původním jazyce

Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 A degrees C and showed reproducible transfection efficiencies in vitro inHEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.

Název v anglickém jazyce

Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Popis výsledku anglicky

Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 A degrees C and showed reproducible transfection efficiencies in vitro inHEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biotechnology Letters

ISSN

0141-5492

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

557-565

Kód UT WoS článku

000350394800010

EID výsledku v databázi Scopus

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