In vitro and in vivo assessment of chitosan modified urocanic acid as gene carrier

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10361546" target="_blank" >RIV/00216208:11110/17:10361546 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064211:_____/17:W0000047

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.msec.2016.09.024" target="_blank" >http://dx.doi.org/10.1016/j.msec.2016.09.024</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2016.09.024" target="_blank" >10.1016/j.msec.2016.09.024</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro and in vivo assessment of chitosan modified urocanic acid as gene carrier

Popis výsledku v původním jazyce

Chitosan nanoparticles modified with 10 and 30% urocanic acid (CUA) via carbodiimide crosslinking were examined for an efficient gene delivery carrier. The CUA gene carrier was characterized by FTIR, TEM, SEM and the in vitro transfection efficiency CUA polyplex was tested with HeLa and 3T3 cells. The loading efficiency of CUA complexes with DNA was assessed at different N/P ratio of 1, 2, 4, 6, 8, and 10. The DNA loading efficiency was found be to &gt;85% for chitosan, CUA10 and CUA30% and the DNA protection ability of CUAl 0 and CUA30 nanoparticle complexes was confirmed upon incubation with Nhel and HindIII. The cell toxicity and cell viability results have supported the non-toxic nature of CUAl 0 and CUA30 nanoparticles. In vitro transfection efficiency of CUAl 0 and CUA30 polyplex was tested for EGFP expression in 3T3 and HeLa cells and a relative maximum % transfection of about 10% was confirmed by CUAl 0 and CUA30 after 96 h transfection. The feasibility and biocompatibility of CUA gene carrier in transgenic chickens was also demonstrated. The in vitro transfection and in vivo embryonic viability studies further confirmed the CUA as promising gene carrier because of the improved biocompatibility and DNA protection ability.

Název v anglickém jazyce

In vitro and in vivo assessment of chitosan modified urocanic acid as gene carrier

Popis výsledku anglicky

Chitosan nanoparticles modified with 10 and 30% urocanic acid (CUA) via carbodiimide crosslinking were examined for an efficient gene delivery carrier. The CUA gene carrier was characterized by FTIR, TEM, SEM and the in vitro transfection efficiency CUA polyplex was tested with HeLa and 3T3 cells. The loading efficiency of CUA complexes with DNA was assessed at different N/P ratio of 1, 2, 4, 6, 8, and 10. The DNA loading efficiency was found be to &gt;85% for chitosan, CUA10 and CUA30% and the DNA protection ability of CUAl 0 and CUA30 nanoparticle complexes was confirmed upon incubation with Nhel and HindIII. The cell toxicity and cell viability results have supported the non-toxic nature of CUAl 0 and CUA30 nanoparticles. In vitro transfection efficiency of CUAl 0 and CUA30 polyplex was tested for EGFP expression in 3T3 and HeLa cells and a relative maximum % transfection of about 10% was confirmed by CUAl 0 and CUA30 after 96 h transfection. The feasibility and biocompatibility of CUA gene carrier in transgenic chickens was also demonstrated. The in vitro transfection and in vivo embryonic viability studies further confirmed the CUA as promising gene carrier because of the improved biocompatibility and DNA protection ability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30212 - Surgery

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Science &amp; Engineering C

ISSN

0928-4931

e-ISSN

—

Svazek periodika

70

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

599-606

Kód UT WoS článku

000388046900071

EID výsledku v databázi Scopus

2-s2.0-84988637998