The Benefits of Ultra-High-Performance Supercritical Fluid Chromatography in Determination of Lipophilic Vitamins in Dietary Supplements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F19%3A10400887" target="_blank" >RIV/00216208:11160/19:10400887 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=sVJYveQoBF" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=sVJYveQoBF</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-018-3666-2" target="_blank" >10.1007/s10337-018-3666-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Benefits of Ultra-High-Performance Supercritical Fluid Chromatography in Determination of Lipophilic Vitamins in Dietary Supplements

Popis výsledku v původním jazyce

Nine tocopherol derivatives including four tocopherols, four tocotrienols, and one esterified form of -tocopherol acetate were analyzed using ultra-high-performance supercritical fluid chromatography (UHPSFC) with diode array detection. The separation was carried out on BEH 2-EP column using shallow gradient elution of 1-4% methanol and CO2 in 5min. Complementary ultra-high-performance liquid chromatography method was developed using Ascentis Express F5 core-shell stationary phase and isocratic elution with 75% acetonitrile/methanol (1:1) and 25% water. This separation took 12min that is the shortest liquid chromatography run in analysis of all tocopherol derivatives reported so far. However, our UHPSFC method resulted in more than two times faster analysis, and allowed direct injection of heptane solutions and extracts from liquid-liquid extraction. These features made UHPSFC a method of choice for subsequent analysis of dietary supplements containing different forms of vitamin E. Sample preparation methods were optimized for individual dosage forms of drops, capsules, tablets, and granulate.

Název v anglickém jazyce

The Benefits of Ultra-High-Performance Supercritical Fluid Chromatography in Determination of Lipophilic Vitamins in Dietary Supplements

Popis výsledku anglicky

Nine tocopherol derivatives including four tocopherols, four tocotrienols, and one esterified form of -tocopherol acetate were analyzed using ultra-high-performance supercritical fluid chromatography (UHPSFC) with diode array detection. The separation was carried out on BEH 2-EP column using shallow gradient elution of 1-4% methanol and CO2 in 5min. Complementary ultra-high-performance liquid chromatography method was developed using Ascentis Express F5 core-shell stationary phase and isocratic elution with 75% acetonitrile/methanol (1:1) and 25% water. This separation took 12min that is the shortest liquid chromatography run in analysis of all tocopherol derivatives reported so far. However, our UHPSFC method resulted in more than two times faster analysis, and allowed direct injection of heptane solutions and extracts from liquid-liquid extraction. These features made UHPSFC a method of choice for subsequent analysis of dietary supplements containing different forms of vitamin E. Sample preparation methods were optimized for individual dosage forms of drops, capsules, tablets, and granulate.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/EF16_019%2F0000841" target="_blank" >EF16_019/0000841: Zvýšení účinnosti a bezpečnosti léčiv a nutraceutik: moderní metody - nové výzvy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

—

Svazek periodika

82

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

477-487

Kód UT WoS článku

000456645900039

EID výsledku v databázi Scopus

2-s2.0-85057576324