In vitro assessment of polyethylene glycol and polyvinylpyrrolidone as hydrophilic additives on bioseparation by polysulfone membranes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F20%3A10417469" target="_blank" >RIV/00216208:11160/20:10417469 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GnatlaOWBV" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GnatlaOWBV</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10853-019-04123-1" target="_blank" >10.1007/s10853-019-04123-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro assessment of polyethylene glycol and polyvinylpyrrolidone as hydrophilic additives on bioseparation by polysulfone membranes

Popis výsledku v původním jazyce

Considering the continuous increment of morbidity in patients with renal disease, the improvement of polymeric membranes characteristics for haemodialysis is a challenging issue. The efficient removal of middle size uremic toxins, as well as biocompatibility is crucial characteristics to ameliorate chronic kidney disease patients&apos; outcomes. In this study, polysulfone membranes with different hydrophilic additives, molecular weight and concentrations were prepared by spin coating method, followed by phase inversion via immersion precipitation. The removal of urea, lysozyme and bovine serum albumin was studied using a miniaturized flow system, mimicking haemodialysis conditions. The optimal composition was 15.0 wt% of polysulfone and 2.5 wt% of polyvinylpyrrolidone K30. This selected membrane was fully permeable for urea, showed up to 50% rejection of lysozyme and very high rejection of albumin. The additive PVP K30 played important role in the improvement of porosity, without compromising the mechanical strength of the membrane. The biocompatibility of membrane was evaluated by material-induced haemolysis and platelet activation tests. The platelet activation and haemolysis were negligible, compared to other biocompatible materials. These results confirmed the haemocompatibility of the optimized membrane. Therefore, the membrane is not expected to modulate the biological response, when used for the haemodialysis treatment.

Název v anglickém jazyce

In vitro assessment of polyethylene glycol and polyvinylpyrrolidone as hydrophilic additives on bioseparation by polysulfone membranes

Popis výsledku anglicky

Considering the continuous increment of morbidity in patients with renal disease, the improvement of polymeric membranes characteristics for haemodialysis is a challenging issue. The efficient removal of middle size uremic toxins, as well as biocompatibility is crucial characteristics to ameliorate chronic kidney disease patients&apos; outcomes. In this study, polysulfone membranes with different hydrophilic additives, molecular weight and concentrations were prepared by spin coating method, followed by phase inversion via immersion precipitation. The removal of urea, lysozyme and bovine serum albumin was studied using a miniaturized flow system, mimicking haemodialysis conditions. The optimal composition was 15.0 wt% of polysulfone and 2.5 wt% of polyvinylpyrrolidone K30. This selected membrane was fully permeable for urea, showed up to 50% rejection of lysozyme and very high rejection of albumin. The additive PVP K30 played important role in the improvement of porosity, without compromising the mechanical strength of the membrane. The biocompatibility of membrane was evaluated by material-induced haemolysis and platelet activation tests. The platelet activation and haemolysis were negligible, compared to other biocompatible materials. These results confirmed the haemocompatibility of the optimized membrane. Therefore, the membrane is not expected to modulate the biological response, when used for the haemodialysis treatment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/EF16_019%2F0000841" target="_blank" >EF16_019/0000841: Zvýšení účinnosti a bezpečnosti léčiv a nutraceutik: moderní metody - nové výzvy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Materials Science

ISSN

0022-2461

e-ISSN

—

Svazek periodika

55

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

1292-1307

Kód UT WoS článku

000490838300002

EID výsledku v databázi Scopus

2-s2.0-85074198502