Polyamine metabolism during the cell cycle of synchronized tobacco BY-2 cell line

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F09%3A10000392" target="_blank" >RIV/00216208:11310/09:10000392 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/09:00335900 RIV/61389030:_____/09:00335900

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polyamine metabolism during the cell cycle of synchronized tobacco BY-2 cell line

Popis výsledku v původním jazyce

The time courses of the contents of free, soluble and insoluble polyamine (PA) conjugates, PA biosynthetic and catabolic enzyme activities and mRNA levels of PA biosynthetic genes were monitored during the cell cycle of synchronized tobacco BY-2 cell line (Nicotiana tabacum L. cv. Bright Yellow 2). Progression through the cell cycle was characterized by specific biphasic changes of PA levels. The first, moderate peak in the amount of free PAs coincided with the S-phase. After a transient decline in G2 phase the contents of free PAs increased rapidly and peaked again during G2/M interface. Then sharply decreased with the minimum at the end of mitosis and during M/G1 transition and started to rise again with the next replication phase. Levels of PA soluble conjugates paralleled those of the free forms. Biosynthetic enzyme activities followed the biphasic manner analogous to the levels of free PAs and seemed to be regulated on both transcriptional and (post)translational level.

Název v anglickém jazyce

Polyamine metabolism during the cell cycle of synchronized tobacco BY-2 cell line

Popis výsledku anglicky

The time courses of the contents of free, soluble and insoluble polyamine (PA) conjugates, PA biosynthetic and catabolic enzyme activities and mRNA levels of PA biosynthetic genes were monitored during the cell cycle of synchronized tobacco BY-2 cell line (Nicotiana tabacum L. cv. Bright Yellow 2). Progression through the cell cycle was characterized by specific biphasic changes of PA levels. The first, moderate peak in the amount of free PAs coincided with the S-phase. After a transient decline in G2 phase the contents of free PAs increased rapidly and peaked again during G2/M interface. Then sharply decreased with the minimum at the end of mitosis and during M/G1 transition and started to rise again with the next replication phase. Levels of PA soluble conjugates paralleled those of the free forms. Biosynthetic enzyme activities followed the biphasic manner analogous to the levels of free PAs and seemed to be regulated on both transcriptional and (post)translational level.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/IAA500200719" target="_blank" >IAA500200719: Formy gama-tubulinu a jejich interakce s proteiny významnými v cytokinezi, při kontrole buněčného cyklu a v polaritě buněk.</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant physiology and biochemistry

ISSN

0981-9428

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000267194800005

EID výsledku v databázi Scopus

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