Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033075" target="_blank" >RIV/61389030:_____/03:56033075 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots.

Popis výsledku v původním jazyce

2-Aminoindan-2-phosphonic acid (AIP), a potent inhibitor of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), was applied to highly synchronous meristematic cells of Vicia faba roots containing cells in G1/S and G2/M cell cycle checkpoints. The inhibition resulted in a slight decline of the content of hydroxycinnamic derivatives 3.5 h after the AIP application. Flow cytometric analyses and BrdU immunolabelling of replicated cells showed that AIP applied in the G1-phase of the cell cycle delayed the progression through S-phase. The transient delay in DNA replication in treated cells was accompanied by accumulation of free spermidine and perchloric acid (PCA)-soluble spermidine conjugates. The cell cycle checkpoint G2/M appeared to be less sensitive to theinhibitor than G1/S. Mitotic activity of asynchronous root meristems was decreased only after the highest tested AIP concentration was applied (100 M) for 24 h. Significant increase in free putrescine and perchloric acid soluble-putrescin

Název v anglickém jazyce

Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots.

Popis výsledku anglicky

2-Aminoindan-2-phosphonic acid (AIP), a potent inhibitor of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), was applied to highly synchronous meristematic cells of Vicia faba roots containing cells in G1/S and G2/M cell cycle checkpoints. The inhibition resulted in a slight decline of the content of hydroxycinnamic derivatives 3.5 h after the AIP application. Flow cytometric analyses and BrdU immunolabelling of replicated cells showed that AIP applied in the G1-phase of the cell cycle delayed the progression through S-phase. The transient delay in DNA replication in treated cells was accompanied by accumulation of free spermidine and perchloric acid (PCA)-soluble spermidine conjugates. The cell cycle checkpoint G2/M appeared to be less sensitive to theinhibitor than G1/S. Mitotic activity of asynchronous root meristems was decreased only after the highest tested AIP concentration was applied (100 M) for 24 h. Significant increase in free putrescine and perchloric acid soluble-putrescin

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A081" target="_blank" >LN00A081: Signální dráhy u rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Science

ISSN

0032-0919

e-ISSN

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Svazek periodika

164

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

10

Strana od-do

823-832

Kód UT WoS článku

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EID výsledku v databázi Scopus

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