Genome mining for the discovery of new nitrilases in filamentous fungi

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10062490" target="_blank" >RIV/00216208:11310/11:10062490 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/11:00360696

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10529-010-0421-7" target="_blank" >http://dx.doi.org/10.1007/s10529-010-0421-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10529-010-0421-7" target="_blank" >10.1007/s10529-010-0421-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genome mining for the discovery of new nitrilases in filamentous fungi

Popis výsledku v původním jazyce

Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expresse in Escherichia coli. This is the first heterologous expression of fungalenzymes of this type. The recombinant enzyme derived from G. Moniliformis was an aromatic nitrilase with an activity of 390 U l-1 culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A.niger and N. crassa that had activities of 2500 and 2700 U l-1 culture, respectively, with phenylacetonitrile as substrate.

Název v anglickém jazyce

Genome mining for the discovery of new nitrilases in filamentous fungi

Popis výsledku anglicky

Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expresse in Escherichia coli. This is the first heterologous expression of fungalenzymes of this type. The recombinant enzyme derived from G. Moniliformis was an aromatic nitrilase with an activity of 390 U l-1 culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A.niger and N. crassa that had activities of 2500 and 2700 U l-1 culture, respectively, with phenylacetonitrile as substrate.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biotechnology Letters

ISSN

0141-5492

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

309-312

Kód UT WoS článku

000286198700014

EID výsledku v databázi Scopus

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