Cyclofructan 6 based stationary phases for hydrophilic interaction liquid chromatography
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10089768" target="_blank" >RIV/00216208:11310/11:10089768 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.chroma.2010.11.027" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2010.11.027</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2010.11.027" target="_blank" >10.1016/j.chroma.2010.11.027</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Cyclofructan 6 based stationary phases for hydrophilic interaction liquid chromatography
Popis výsledku v původním jazyce
New stationary phases for hydrophilic interaction liquid chromatography (HILIC) were synthesized by covalently attaching native cyclofructan 6 (CF6) to silica gel. The chromatographic characteristics of the new stationary phases were evaluated and compared to three different types of commercial HILIC columns. The CF6 columns produced considerably different retention and selectivity patterns for various classes of polar analytes, including nucleic acid compounds, xanthines, beta-blockers, salicylic acidand its derivatives, and maltooligosaccharides. Univariate optimization approaches were examined including organic modifier (acetonitrile) contents and buffer pH and salt concentration. The thermodynamic characteristic of the CF6 stationary phase was investigated by considering the column temperature effect on retention and utilizing van't Hoff plots. CF6 based stationary phases appear to have exceptionally broad applicability for HILIC mode separations.
Název v anglickém jazyce
Cyclofructan 6 based stationary phases for hydrophilic interaction liquid chromatography
Popis výsledku anglicky
New stationary phases for hydrophilic interaction liquid chromatography (HILIC) were synthesized by covalently attaching native cyclofructan 6 (CF6) to silica gel. The chromatographic characteristics of the new stationary phases were evaluated and compared to three different types of commercial HILIC columns. The CF6 columns produced considerably different retention and selectivity patterns for various classes of polar analytes, including nucleic acid compounds, xanthines, beta-blockers, salicylic acidand its derivatives, and maltooligosaccharides. Univariate optimization approaches were examined including organic modifier (acetonitrile) contents and buffer pH and salt concentration. The thermodynamic characteristic of the CF6 stationary phase was investigated by considering the column temperature effect on retention and utilizing van't Hoff plots. CF6 based stationary phases appear to have exceptionally broad applicability for HILIC mode separations.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
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Svazek periodika
1218
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
10
Strana od-do
270-279
Kód UT WoS článku
000286700600012
EID výsledku v databázi Scopus
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