Utilization of dual retention mechanism on columns with bonded PEG and diol stationary phases for adjusting the separation selectivity of phenolic and flavone natural antioxidants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F09%3A00008423" target="_blank" >RIV/00216275:25310/09:00008423 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of dual retention mechanism on columns with bonded PEG and diol stationary phases for adjusting the separation selectivity of phenolic and flavone natural antioxidants

Popis výsledku v původním jazyce

The retention of phenolic acid and flavone antioxidants was investigated on three columns with bonded PEG, hydroxy (DIOL) and mixed oxyethylene-hydroxy (Luna HILIC) stationary phases in buffered aqueous ACN mobile phases. All columns show mixed retentionmechanism: reversed phases in highly aqueous mobile phases and normal phases (HILIC, hydrophilic interaction LC) in mobile phases with high concentration of ACN, showing characteristic U-shape retention versus mobile phase composition plots. Equations enabling description and prediction of retention of sample compounds over the full mobile phase composition range were compared. Dual retention mechanism offers possibilities for using differences in selectivity between the two separation modes on a single column to improve the separation of natural antioxidants. The Luna HILIC column shows larger HILIC retention range, enhanced retention, improved selectivity and better peak symmetry in comparison to the PEG and DIOL columns.

Název v anglickém jazyce

Utilization of dual retention mechanism on columns with bonded PEG and diol stationary phases for adjusting the separation selectivity of phenolic and flavone natural antioxidants

Popis výsledku anglicky

The retention of phenolic acid and flavone antioxidants was investigated on three columns with bonded PEG, hydroxy (DIOL) and mixed oxyethylene-hydroxy (Luna HILIC) stationary phases in buffered aqueous ACN mobile phases. All columns show mixed retentionmechanism: reversed phases in highly aqueous mobile phases and normal phases (HILIC, hydrophilic interaction LC) in mobile phases with high concentration of ACN, showing characteristic U-shape retention versus mobile phase composition plots. Equations enabling description and prediction of retention of sample compounds over the full mobile phase composition range were compared. Dual retention mechanism offers possibilities for using differences in selectivity between the two separation modes on a single column to improve the separation of natural antioxidants. The Luna HILIC column shows larger HILIC retention range, enhanced retention, improved selectivity and better peak symmetry in comparison to the PEG and DIOL columns.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F07%2F0641" target="_blank" >GA203/07/0641: Nové analytické postupy v kombinovaných fázových systémech s vysokou separační kapacitou</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

17

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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