Retention Mechanism Studies of Selected Amino Acids and Vitamin B6 on HILIC Columns with Evaporative Light Scattering Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896423" target="_blank" >RIV/00216275:25310/13:39896423 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs10337-013-2502-y" target="_blank" >http://link.springer.com/article/10.1007%2Fs10337-013-2502-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-013-2502-y" target="_blank" >10.1007/s10337-013-2502-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Retention Mechanism Studies of Selected Amino Acids and Vitamin B6 on HILIC Columns with Evaporative Light Scattering Detection

Popis výsledku v původním jazyce

The goal of the study was to investigate separation mechanism of selected "essential" amino acids (leucine, isoleucine, threonine, tryptophan, proline, and glycine) and vitamin B6 in hydrophilic interaction liquid chromatography (HILIC) with the evaporative light scattering detection. Chromatographic measurements were made on three different HILIC columns: amide-silica (TSK-gel Amide-80), amino-silica (TSK-gel NH2-100), and cross-linked diol (Luna HILIC). The retention behaviour of the analytes was investigated as a function of different binary hydro-organic mobile phases containing 10-90 % (v/v) acetonitrile. The compounds studied were separated under isocratic and gradient conditions. The best results of tested biologically active compounds separation were obtained on the TSK-gel NH2-100 column. TSK-gel NH2 column showed mixed HILIC-ion-exchange mechanism, the highest separation efficiency and better selectivity and resolution for tested analytes than the other studied column, especi

Název v anglickém jazyce

Retention Mechanism Studies of Selected Amino Acids and Vitamin B6 on HILIC Columns with Evaporative Light Scattering Detection

Popis výsledku anglicky

The goal of the study was to investigate separation mechanism of selected "essential" amino acids (leucine, isoleucine, threonine, tryptophan, proline, and glycine) and vitamin B6 in hydrophilic interaction liquid chromatography (HILIC) with the evaporative light scattering detection. Chromatographic measurements were made on three different HILIC columns: amide-silica (TSK-gel Amide-80), amino-silica (TSK-gel NH2-100), and cross-linked diol (Luna HILIC). The retention behaviour of the analytes was investigated as a function of different binary hydro-organic mobile phases containing 10-90 % (v/v) acetonitrile. The compounds studied were separated under isocratic and gradient conditions. The best results of tested biologically active compounds separation were obtained on the TSK-gel NH2-100 column. TSK-gel NH2 column showed mixed HILIC-ion-exchange mechanism, the highest separation efficiency and better selectivity and resolution for tested analytes than the other studied column, especi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

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Svazek periodika

76

Číslo periodika v rámci svazku

15-16

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

929-937

Kód UT WoS článku

000322388200006

EID výsledku v databázi Scopus

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