Phototoxic effect of TPPS(4) and MgTPPS(4) on DNA fragmentation of HeLa cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10105069" target="_blank" >RIV/00216208:11310/11:10105069 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/11:33118366

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.tiv.2010.11.005" target="_blank" >http://dx.doi.org/10.1016/j.tiv.2010.11.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.tiv.2010.11.005" target="_blank" >10.1016/j.tiv.2010.11.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phototoxic effect of TPPS(4) and MgTPPS(4) on DNA fragmentation of HeLa cells

Popis výsledku v původním jazyce

Photodynamic therapy (PDT) is an alternative method of tumour treatment. It is based on a photochemical reaction of a photosensitizer, irradiation, and O(2) which converts to cytotoxic (1)O(2) and other forms of reactive oxygen species (ROS). The comet assay (also called single-cell gel electrophoresis. SCGE) is a sensitive, simple and quantitative technique for detection of DNA damage. In our study we investigated the phototoxicity of the two porphyrin photosensitizers. TPPS4 and MgTPPS4, on HeLa cells. Three different radiation doses and six different concentrations of the photosensitizers were used. Our results show that the DNA of the cells treated with the TPPS4 and MgTPPS4 at the concentrations higher than 51 mu M was highly fragmented indicatinga strong phototoxic effect resulting in a cell apoptosis. On the base of our results we can hypothesize that even the irradiation dose of 1 J cm(-2) is sufficient enough to provoke the DNA fragmentation.

Název v anglickém jazyce

Phototoxic effect of TPPS(4) and MgTPPS(4) on DNA fragmentation of HeLa cells

Popis výsledku anglicky

Photodynamic therapy (PDT) is an alternative method of tumour treatment. It is based on a photochemical reaction of a photosensitizer, irradiation, and O(2) which converts to cytotoxic (1)O(2) and other forms of reactive oxygen species (ROS). The comet assay (also called single-cell gel electrophoresis. SCGE) is a sensitive, simple and quantitative technique for detection of DNA damage. In our study we investigated the phototoxicity of the two porphyrin photosensitizers. TPPS4 and MgTPPS4, on HeLa cells. Three different radiation doses and six different concentrations of the photosensitizers were used. Our results show that the DNA of the cells treated with the TPPS4 and MgTPPS4 at the concentrations higher than 51 mu M was highly fragmented indicatinga strong phototoxic effect resulting in a cell apoptosis. On the base of our results we can hypothesize that even the irradiation dose of 1 J cm(-2) is sufficient enough to provoke the DNA fragmentation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology in Vitro

ISSN

0887-2333

e-ISSN

—

Svazek periodika

25

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

1169-1172

Kód UT WoS článku

000294143700003

EID výsledku v databázi Scopus

—