Polysome Analysis and RNA Purification from Sucrose Gradients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10107312" target="_blank" >RIV/00216208:11310/11:10107312 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/11:00373375

Výsledek na webu

<a href="http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-59745-248-9_20" target="_blank" >http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-59745-248-9_20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-59745-248-9_20" target="_blank" >10.1007/978-1-59745-248-9_20</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polysome Analysis and RNA Purification from Sucrose Gradients

Popis výsledku v původním jazyce

Velocity separation of translation complexes in linear sucrose gradients is the ultimate method for both analysis of the overall fitness of protein synthesis as well as for detailed investigation of physiological roles played by individual factors of thetranslational machinery. Polysome profile analysis is a frequently performed task in translational control research that not only enables direct monitoring of the efficiency of translation but can easily be extended with a wide range of downstream applications such as Northern and Western blotting, genome-wide microarray analysis or qRT-PCR. This chapter provides a basic overview of the polysome profile analysis technique and the RNA isolation procedure from sucrose gradients. We also discuss possibleexperimental pitfalls of data normalization, describe main alternatives of the basic protocol and outline a novel application of denaturing RNA electrophoresis in several steps of polysome profile analysis.

Název v anglickém jazyce

Polysome Analysis and RNA Purification from Sucrose Gradients

Popis výsledku anglicky

Velocity separation of translation complexes in linear sucrose gradients is the ultimate method for both analysis of the overall fitness of protein synthesis as well as for detailed investigation of physiological roles played by individual factors of thetranslational machinery. Polysome profile analysis is a frequently performed task in translational control research that not only enables direct monitoring of the efficiency of translation but can easily be extended with a wide range of downstream applications such as Northern and Western blotting, genome-wide microarray analysis or qRT-PCR. This chapter provides a basic overview of the polysome profile analysis technique and the RNA isolation procedure from sucrose gradients. We also discuss possibleexperimental pitfalls of data normalization, describe main alternatives of the basic protocol and outline a novel application of denaturing RNA electrophoresis in several steps of polysome profile analysis.

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

RNA Methods and Protocols

ISBN

978-1-58829-913-0

Počet stran výsledku

17

Strana od-do

293-309

Počet stran knihy

344

Název nakladatele

Springer Science + Business Media (Humana Press)

Místo vydání

New York

Kód UT WoS kapitoly

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