Determination of N-methyl-2-pyrrolidone and its metabolites in urine by micellar electrokinetic chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10118931" target="_blank" >RIV/00216208:11310/11:10118931 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2478/s11532-011-0062-2" target="_blank" >http://dx.doi.org/10.2478/s11532-011-0062-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11532-011-0062-2" target="_blank" >10.2478/s11532-011-0062-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of N-methyl-2-pyrrolidone and its metabolites in urine by micellar electrokinetic chromatography

Popis výsledku v původním jazyce

A fast and accurate micellar electrokinetic capillary chromatography (MEKC) method was developed for monitoring N-methyl-2-pyrrolidone (NMP) exposure. Baseline separation of NMP and its main metabolites: 5-hydroxy-N-methyl-2-pyrrolidone (5HNMP), N-methylsuccinimide (MSI), 2-hydroxy-N-methylsuccinimide (2HMSI), and 2-pyrrolidone (2P) was obtained within 6 min in an uncoated fused silica capillary using 5 mM phosphate buffer and 140 mM sodium dodecyl sulfate (pH 7.1) as background electrolyte (BGE). On-line UV-detection was performed at 200 nm and the applied electric field was 400 V cm(-1). Possible interference of BGE-induced system peaks on separation was investigated by computer simulation and no such interference was observed. The developed MEKC method combined with solid phase extraction for sample preparation was successfully applied to the analysis of urine of rats exposed to NMP. The urinary excretion was determined in 0-6 h and 6-24 h specimens collected after an intragastic ad

Název v anglickém jazyce

Determination of N-methyl-2-pyrrolidone and its metabolites in urine by micellar electrokinetic chromatography

Popis výsledku anglicky

A fast and accurate micellar electrokinetic capillary chromatography (MEKC) method was developed for monitoring N-methyl-2-pyrrolidone (NMP) exposure. Baseline separation of NMP and its main metabolites: 5-hydroxy-N-methyl-2-pyrrolidone (5HNMP), N-methylsuccinimide (MSI), 2-hydroxy-N-methylsuccinimide (2HMSI), and 2-pyrrolidone (2P) was obtained within 6 min in an uncoated fused silica capillary using 5 mM phosphate buffer and 140 mM sodium dodecyl sulfate (pH 7.1) as background electrolyte (BGE). On-line UV-detection was performed at 200 nm and the applied electric field was 400 V cm(-1). Possible interference of BGE-induced system peaks on separation was investigated by computer simulation and no such interference was observed. The developed MEKC method combined with solid phase extraction for sample preparation was successfully applied to the analysis of urine of rats exposed to NMP. The urinary excretion was determined in 0-6 h and 6-24 h specimens collected after an intragastic ad

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Central European Journal of Chemistry

ISSN

1895-1066

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

9

Strana od-do

825-833

Kód UT WoS článku

000293020400008

EID výsledku v databázi Scopus

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